Expression Evaluation of CD2AP, ITGA3, and ITGB1 in Podocyte Cell Culture After Albumin Overload with and Without Puromycin-Aminoglycoside Damage

Le résumé fourni par la source

Background: Proteinuria is considered a prognostic marker for kidney disease and one of the main symptoms of podocytopathies. Several molecules participate in the maintenance of glomerular filtration barrier, including the CD2-associated protein (CD2AP) present in the slit diaphragm and the α3β1 integrin in the basal membrane of podocytes. This study aimed to evaluate the relative expression of CD2AP, ITGA3 and ITGB1 genes after overload of albumin in podocytes in vitro. Methods: Conditionally immortalized human podocytes were exposed to progressive concentrations of albumin (0, 3, 20 and 40 mg/ml) for 24 hours under two conditions: with or without 12 hours of 15 ug/uL of puromycin aminoglycoside (PAN) treatment. We evaluated CD2AP, ITGA3 and ITGB1 genes relative expression through qPCR (n=3). The Friedman test was used to compare the different albumin exposures with and without PAN (p<0.05). Results: The fold change of CD2AP, ITGA3 and ITGB1 genes are presented in Table 1. No significant difference was found among the albumin exposures, neither with nor without PAN (p>0.05). Conclusions: In the condition here studied, albumin overload have not modulated CD2AP, ITGA3 and ITGB1 genes. Funding: Government Support - Non-U.S. Fold change of CD2AP, ITGA3 and ITGB1 genes - PAN treatment Albumin exposure (mg/mL) Fold Change CD2AP Fold Change ITGA3 Fold Change ITGB1 No 0 1 1 1 3 0.017418 1.15284961 0.938208375 20 0.044871 1.423730085 1.388398524 40 0.02611 0.516405126 0.891691752 Yes 0 1 1 1 3 1.386209 1.241183205 1.258075685 20 1.776701 1.798257843 1.941487235 40 1.896176 0.982406017 1.587184907

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.