Distribution of toxigenic cyanobacteria in Alpine lakes and rivers as revealed by molecular screening
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Le résumé fourni par la source
The increasing frequency of cyanobacteria blooms in waterbodies caused by ecosystem eutrophication could endanger human health. This risk can be mitigated by effective monitoring incorporating molecular methods. To date, most molecular studies on toxigenic cyanobacteria have been limited to microcystins (MCs), disregarding other cyanotoxins, to freshwater planktic habitats while ignoring benthic habitats, and to limited geographic areas (usually one or a few specific waterbodies). In this study, we used PCR-based methods including PCR product sequencing and chemical-analytical methods (LC-MS/MS) to screen many plankton (n = 123) and biofilm samples (n = 113) originating from 29 Alpine lakes and 18 rivers for their cyanotoxin production potential. Both mcyE (indicating MC synthesis) and anaC (indicating anatoxin (ATX) synthesis) gene fragments were able to qualitatively predict MC or ATX occurrence. The abundance of mcyE gene fragments was significantly related to MC concentrations in plankton samples (R2 = 0.61). mcyE gene fragments indicative of MC synthesis were most abundant in planktic samples (65%) and were assigned to the genera Planktothrix and Microcystis. However, mcyE rarely occurred in biofilms of lakes and rivers, i.e., 4% and 5%, respectively, and were assigned to Microcystis, Planktothrix, and Nostoc. In contrast, anaC gene fragments occurred frequently in planktic samples (14% assigned to Tychonema, Phormidium (Microcoleus), and Oscillatoria), but also in biofilms of lakes (49%) and rivers (18%) and were assigned to the genera Phormidium, Oscillatoria, and Nostocales. The cyrJ gene fragment indicating cylindrospermopsin synthesis occurred only once in plankton (assigned to Dolichospermum), while saxitoxin synthesis potential was not detected. For plankton samples, monomictic and less eutrophic conditions were positively related to mcyE/MC occurrence frequency, while oligomictic conditions were related to anaC/ATX frequency. The anaC/ATX frequency in biofilm was related to the lake habitats generally showing higher biodiversity as revealed from metabarcoding in a parallel study.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Distribution of toxigenic cyanobacteria in Alpine lakes and rivers as revealed by molecular screening
- Date Crossref
- 01/07/2024
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Ljubljana National Institute of Biology pays non établi dans la noticeUniversité ou école supérieure
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Universität Innsbruck pays non établi dans la noticeUniversité ou école supérieure
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National Institute of Biology pays non établi dans la noticeStructure de recherche
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Fondazione Edmund Mach pays non établi dans la noticeInstitution
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University of Applied Sciences and Arts of Southern Switzerland Institute of Earth Sciences pays non établi dans la noticeUniversité ou école supérieure
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Istituto Superiore per la Protezione e la Ricerca Ambientale pays non établi dans la noticeOrganisme public
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Slovenian Environment Agency pays non établi dans la noticeOrganisme public
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German Environment Agency pays non établi dans la noticeOrganisme public
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University of Palermo pays non établi dans la noticeUniversité ou école supérieure
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Research Department for Limnology pays non établi dans la noticeInstitution
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Research and Innovation Centre pays non établi dans la noticeInstitution
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Italian Institute for Environmental Protection and Research (ISPRA) pays non établi dans la noticeStructure de recherche
National Institute of Biology — University of Ljubljana, Universität Innsbruck et National Institute of Biology, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.