PD59-07 SACRAL NEUROMODULATION DEVICE BIOFILMS HARBOR RIFAMPIN AND MINOCYCLINE RESISTANCE GENES AND ARE RECONSTITUTABLE ON TITANIUM, CERAMIC, AND OTHER MEDICAL MATERIALS

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You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Neuromodulation (PD59)1 May 2024PD59-07 SACRAL NEUROMODULATION DEVICE BIOFILMS HARBOR RIFAMPIN AND MINOCYCLINE RESISTANCE GENES AND ARE RECONSTITUTABLE ON TITANIUM, CERAMIC, AND OTHER MEDICAL MATERIALS Glenn Werneburg, Daniel Hettel, Sandip Vasavada, Sromona Mukherjee, Howard Goldman, Ava Adler, Bradley Gill, Raymond Rackley, Jacqueline Zillioux, Sarah Martin, Daniel Shoskes, and Aaron Miller Glenn WerneburgGlenn Werneburg , Daniel HettelDaniel Hettel , Sandip VasavadaSandip Vasavada , Sromona MukherjeeSromona Mukherjee , Howard GoldmanHoward Goldman , Ava AdlerAva Adler , Bradley GillBradley Gill , Raymond RackleyRaymond Rackley , Jacqueline ZilliouxJacqueline Zillioux , Sarah MartinSarah Martin , Daniel ShoskesDaniel Shoskes , and Aaron MillerAaron Miller View All Author Informationhttps://doi.org/10.1097/01.JU.0001009544.34256.8a.07AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Sacral neuromodulation (SNM) is effective therapy for medically-refractory overactive bladder and urinary retention. A subset of SNM devices requires surgical removal for device-associated infection. We sought to assess sacral neuromodulation device biofilms for the presence of genes coding for resistance to commonly used antibiotics including rifampin and minocycline. Further, we sought to reconstitute biofilm formation in vitro using bioreactor, designed to mimic human tissue with a medical device present. We hypothesized that SNM device biofilms would frequently harbor resistance genes to clinically-relevant antibiotics, and reconstitute differentially based on medical device material type. METHODS: Patients scheduled to undergo SNM device removal or revision were consented per IRB-approved protocol. Device biofilms and controls were swabbed intraoperatively upon device exposure. Nucleotides were isolated and subjected to RT-PCR to detect the genes for tetA (tetracycline/minocycline resistance), and rpoB (rifampin resistance) alongside controls. Reconstitution of biofilm of isolated bacteria from explanted devices was performed and compared (two-tailed t-test) using a continuous flow stir tank bioreactor on a series of relevant medical device materials. RESULTS: 37 devices were included. 24% of biofilms harbored a tetA minocycline resistance gene and 53% harbored a rpoB rifampin resistance gene. Biofilms from isolated strains were consistently reconstituted in vitro including Staphylococcus aureus (Figure 1) and S. epidermidis, as assessed by scanning electron microscopy (Figure 1 panel A) and plate count assay (Figure 1 panel B). Biofilm formation differed by microbial strain (p<0.001) and material type (p<0.05). CONCLUSIONS: We commonly detected genes coding for antibiotic resistance to minocycline and rifampin, antibiotics used in commercially available formulations designed to reduce infectious risk in SNM and other medical devices. We also demonstrated biofilm reconstitution of microbial strains isolated from SNM devices, which provides new opportunities to test novel device materials and coatings. Download PPT Source of Funding: SUFU Care Foundation Neuromodulation Award © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1224 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Glenn Werneburg More articles by this author Daniel Hettel More articles by this author Sandip Vasavada More articles by this author Sromona Mukherjee More articles by this author Howard Goldman More articles by this author Ava Adler More articles by this author Bradley Gill More articles by this author Raymond Rackley More articles by this author Jacqueline Zillioux More articles by this author Sarah Martin More articles by this author Daniel Shoskes More articles by this author Aaron Miller More articles by this author Expand All Advertisement PDF downloadLoading ...

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