Additional file 10 of Transcriptome analyses of liver in newly-hatched chicks during the metabolic perturbation of fasting and re-feeding reveals THRSPA as the key lipogenic transcription factor

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Additional file 10: Information on primers used for qRT-PCR verification of differential gene expression. A Microsoft Excel file containing a single worksheet “Primers qRT-PCR Assay”. This table provides information on the design of qRT-PCR primers for 20 chicken genes. The Roslin Institute Gallus gallus (RIGG) oligo ID number, NCBI Entrez Gene ID, gene symbol, gene description, and the 5′-3′ sequence for forward and reverse primers used for qRT-PCR analysis are provided. Although four “invariant genes” were included, only two genes were acceptable by qBase software for use in normalization of qRT-PCR expression levels.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.