Impact of Radiation on Rice Calli and Subsequent In vitro Regeneration

Résumé fourni par la source

Aromatic rice has high commercial importance with greater economic return. Among the aromatic rice varieties, Kataribhog is a famous local variety that is mostly cultivated in the Dinajpur district northern part of Bangladesh. The yield performance of Kataribhog is lower (<2.50 t/ha) and has shown lodging tendency. Under these circumstances, a research program was taken to improve the existing problem through in vitro culture including nuclear irradiation techniques. Gamma irradiation (Co60) is a powerful tool in crop improvement, and its effects on callus development from kataribhog rice were explored in this research. Three types of media, PT-100G, PT-011G, and PT-100, were utilized to induce callus, which were then subjected to three different doses of radiation (10Gy, 12Gy, and 15Gy). Among the tested media, PT-100G was the most effective, boasting an impressive average of Callus initiation of 79.27% and embryogenic callus induction percentage of 32.93%. In contrast, PT-011G and PT-100 lagged with 52.46% and 44.44% callus initiation; 27.87% and 11.11% embryogenic callus induction, respectively. The radiation dose of 10 Gy was optimal for calli survival, showing an average percentage of 80.00%. 10 Gy is the most effective dose for promoting plant regeneration.

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