EVALUATION OF ANTICANCER ACTIVITY OF PLANT MEDIATED IRON OXIDE NANOPARTICLES USING RHAZYA STRICTA

Le résumé fourni par la source

Green synthesis is an effective method for the synthesis of nanoparticles (NPs), so the objective of this project was to synthesis FeNPs using the crude extract from Raza stricta, a green synthesis approach. After the synthesis different microscopic as well spectroscopic techniques, including XRD, UV/VIS, SEM and EDX were used to confirm the synthesis as well as size and shape of the synthesized FeNPs. The resulting product was found to be 48.32 nm at 2 mM concentration. The synthesized nanoparticles were then tested for their cytotoxicity using Brine shrimps lethality test as in-vitro and human hepatocellular carcinoma cancer lines huH-1 as in-vivo. The brine shrimps lethality (BSL) assays showed concentration-dependent mortality where maximum (43.3%) mortality was observed at 100 µg. mL-1 and minimum mortality (6.7%) at 5 µg. mL-1 after 48 hrs. The IC50 of the FeNPs against brine shrimps was observed as 137.4 µM while the standard Etopside was 33.4 µM after 48 hrs. During the in-vivo cytotoxicity test against huH-1 has confined the non-toxicity behavior of the synthesized FeNPs, where at a maximum concentration (500µg /ml) the FeNPs revealed 52.5% cell viability with 654.8 µM IC50 values. It is clear from the result that the biosynthesized FeNPs using R. stricta possess less cytotoxic and are effectively safe. The in vivo cytotoxicity against huH-1 hepatoma cancer cell line also confined the non-toxicity of synthesized FeNPs where at a maximum concentration (500µg /ml) the FeNPs revealed 52.5% cell viability with 654.8 µM IC50 values. It is concluded that bio-mediated FeNPs were effective against cancer cell lines. Therefore, it is suggested that the bio-mediated NPs are safe and eco-friendly with no toxicity and could have overwhelming applications in health sciences.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.