Functional anaysis of miR-143-3p/KSR2 interaction and oncogenic function in JURKAT and ALL-SIL T-cell acute lymphoblastic leukemia cell lines

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1. FCS files from GFP competition assay performed in ALL-SIL and JURKAT cell lines upon transduction with hsa-mir-143 expression vector (pCDH miR-143-3p) or empty vector (pCDH EV) as control. 2. Uncropped chemiluminescent immunoblot in JURKAT and ALL-SIL cell lines transduced with hsa-mir-143 expression vector (pCDH miR-143-3p) or empty vector (pCDH EV) as control. Upper band is KSR2 protein (~100 kDa) and lower band is loading control GAPDH protein (~37 kDa). Order of samples on the membrane: JURKAT pCDH miR-143-3p replicate 1, pCDH EV replicate 1, pCDH EV replicate 2, pCDH miR-143-3p replicate 2, pCDH EV replicate 3, pCDH miR-143-3p replicate 3, ALL-SIL pCDH miR-143-3p replicate 1, pCDH miR-143-3p replicate 2, pCDH EV replicate 1, pCDH miR-143-3p replicate 3, pCDH EV replicate 2, pCDH EV replicate 3. 3. RT-qPCR amplification data for relative quantification of KSR2 expression in reference to ACTB and GAPDH in JURKAT and ALL-SIL cell lines expressing deadCas9-KRAB system for transcriptional repression, upon transduction with sgRNA targeting KSR2 transcription start site vector (KSR2 sgRNA1 and KSR2 sgRNA2) or non-targeting sgRNA vector (NT) as control. 4. FCS files from GFP competition assay performed in ALL-SIL and JURKAT cell lines expressing deadCas9-KRAB system for transcriptional repression, upon transduction with sgRNA targeting KSR2 transcription start site vector (KSR2 sgRNA1 and KSR2 sgRNA2) or non-targeting sgRNA vector (NT) as control.

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