Raw diffraction images of Transportin-1 in complex with a nuclear localization signal peptide

Le résumé fourni par la source

Transportin-1 is one of the nuclear transport receptors. Transportin-1 binds a PY-NLS sequence of cargo proteins and brings them into the nucleus. We used datasets to investigate the protocol of detecting polymorphs using Hierarchical clustering (Acta D., in preparation). All diffraction data of this protein crystals were collected at BL32XU, SPring-8, using an automated data collection system ZOO. From four crystals, datasets were collected using a continuous helical scan scheme for 720º oscillation with the following experimental parameters; Beam size: 10 µm × 15 µm, Wavelength: 1.0000 Å, Total dose/crystal: 10 MGy, Detector: EIGER X 9M (DECTRIS Co. Ltd.). All crystals belonged to space group C2 with unit cell parameters roughly corresponding to a=154.3, b=153.8, c=74.3 Å, b=101.3°.

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