Pharmacometabolomics and mass spectrometry imaging approach to reveal the neurochemical mechanisms of Polygala tenuifolia
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Le résumé fourni par la source
Polygala tenuifolia, commonly known as Yuanzhi (YZ) in Chinese, has been shown to possess anti-insomnia properties. However, the material basis and the mechanism underlying its sedative-hypnotic effects remain unclear. Herein, we investigated the active components and neurochemical mechanism of YZ extracts using liquid chromatography tandem mass spectrometry (LC-MS/MS)-based pharmacometabolomics and Mass Spectrometry Imaging (MSI)-based spatial resolved metabolomics. According to the results, seventeen prototypes out of 101 ingredients in the YZ extract were detected in both the plasma and brain, which might be the major components contributing to the sedative-hypnotic effects. Network pharmacology analysis revealed that these prototypes may exert their effects through neuroactive ligand-receptor interaction, serotonergic synapse, and dopaminergic synapse, and dopaminergic synapse, among other pathways. LC-MS/MS-based targeted metabolomics and Western blot (WB) revealed that tryptophan-serotonin-melatonin (Trp-5-HT-Mel) and tyrosine-norepinephrine-adrenaline (Tyr-Ne-Ad) are the key regulated pathways. Dopa decarboxylase (DDC) upregulation and phenylethanolamine N-methyltransferase (PNMT) downregulation further confirmed these pathways. Furthermore, MSI-based spatially resolved metabolomics revealed notable alterations in serotonin (5-HT) in the pineal gland, and Ad in the brainstem, including the middle brain, pons, and hypothalamus. In summary, this study illustrates the efficacy of an integrated multidimensional metabolomics approach in unraveling the sedative-hypnotic effects and neurochemical mechanisms of a Chinese herbal medicine, YZ.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Pharmacometabolomics and mass spectrometry imaging approach to reveal the neurochemical mechanisms of Polygala tenuifolia
- Date Crossref
- 01/07/2024
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Minzu University of China Key Laboratory of Mass Spectrometry Imaging and Metabolomics (National Ethnic Affairs Commission) pays non établi dans la noticeUniversité ou école supérieure
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State Ethnic Affairs Commission pays non établi dans la noticeOrganisme public
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Chinese Academy of Medical Sciences & Peking Union Medical College State Key Laboratory of Bioactive Substance and Function of Natural Medicines pays non établi dans la noticeUniversité ou école supérieure
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School of Pharmacy Key Laboratory of Ethnomedicine of Ministry of Education pays non établi dans la noticeUniversité ou école supérieure
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College of Life and Environmental Sciences Center for Imaging and Systems Biology pays non établi dans la noticeUniversité ou école supérieure
Key Laboratory of Mass Spectrometry Imaging and Metabolomics (National Ethnic Affairs Commission) — Minzu University of China, State Ethnic Affairs Commission et State Key Laboratory of Bioactive Substance and Function of Natural Medicines — Chinese Academy of Medical Sciences & Peking Union Medical College, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.