FIGURE 5 from Immune Profiling of Vulvar Squamous Cell Cancer Discovers a Macrophage-rich Subtype Associated with Poor Prognosis
Le résumé fourni par la source
Characterization of in vitro polarized TAMs. A, Cytokines and chemokines detected in the supernatant of high-density VSCC cultures by ELISA. B, Healthy donor monocytes were differentiated into macrophages using GMCSF and exposed to different culture conditions: medium alone (monocyte-derived macrophage), VSCC supernatant (in vitro polarized TAM), or 20 ng/mL IFNγ (M1). Flow cytometry on day 5 determined surface antigen expression, presented as d-gMFIs. C, Macrophages were generated as in B; TNFα and IL10 levels were quantified in the supernatant by ELISA on day 5; background levels were subtracted. Data represent results from two individuals and/or experiments and are presented as mean ± SEM; significance was determined by two-sided Student t test.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- FIGURE 5 from Immune Profiling of Vulvar Squamous Cell Cancer Discovers a Macrophage-rich Subtype Associated with Poor Prognosis
- Date Crossref
- 21/03/2024
- Éditeur
- American Association for Cancer Research (AACR)
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.