A higher‐yield hybrid rice is achieved by assimilating a dominant heterotic gene in inbred parental lines
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Le résumé fourni par la source
Summary The exploitation of heterosis to integrate parental advantages is one of the fastest and most efficient ways of rice breeding. The genomic architecture of heterosis suggests that the grain yield is strongly correlated with the accumulation of numerous rare superior alleles with positive dominance. However, the improvements in yield of hybrid rice have shown a slowdown or even plateaued due to the limited availability of complementary superior alleles. In this study, we achieved a considerable increase in grain yield of restorer lines by inducing an alternative splicing event in a heterosis gene OsMADS1 through CRISPR‐Cas9, which accounted for approximately 34.1%–47.5% of yield advantage over their corresponding inbred rice cultivars. To achieve a higher yield in hybrid rice, we crossed the gene‐edited restorer parents harbouring OsMADS1GW3p6 with the sterile lines to develop new rice hybrids. In two‐line hybrid rice Guang‐liang‐you 676 (GLY676), the yield of modified hybrids carrying the homozygous heterosis gene OsMADS1GW3p6 significantly exceeded that of the original hybrids with heterozygous OsMADS1. Similarly, the gene‐modified F1 hybrids with heterozygous OsMADS1GW3p6 increased grain yield by over 3.4% compared to the three‐line hybrid rice Quan‐you‐si‐miao (QYSM) with the homozygous genotype of OsMADS1. Our study highlighted the great potential in increasing the grain yield of hybrid rice by pyramiding a single heterosis gene via CRISPR‐Cas9. Furthermore, these results demonstrated that the incomplete dominance of heterosis genes played a major role in yield‐related heterosis and provided a promising strategy for breeding higher‐yielding rice varieties above what is currently achievable.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A higher‐yield hybrid rice is achieved by assimilating a dominant heterotic gene in inbred parental lines
- Date Crossref
- 07/03/2024
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Chinese Academy of Sciences pays non établi dans la noticeOrganisme public
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National Center for Gene Research pays non établi dans la noticeStructure de recherche
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Center for Excellence in Molecular Plant Sciences pays non établi dans la noticeUniversité ou école supérieure
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ShanghaiTech University pays non établi dans la noticeUniversité ou école supérieure
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Imperial College London Department of Life Sciences pays non établi dans la noticeUniversité ou école supérieure
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State Key Laboratory of Plant Molecular Genetics National Center for Gene Research pays non établi dans la noticeStructure de recherche
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These authors contributed equally to this work pays non établi dans la noticeInstitution
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School of Life Science and Technology pays non établi dans la noticeUniversité ou école supérieure
Chinese Academy of Sciences, National Center for Gene Research et Center for Excellence in Molecular Plant Sciences, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.