Tissue-resident memory T cells in human kidney transplants have alloreactive potential

Résumé fourni par la source

The extent to which tissue-resident memory T (T RM ) cells in transplanted organs possess alloreactivity is uncertain. This study investigates the alloreactive potential of T RM cells in kidney explants from 4 patients who experienced severe acute rejection leading to graft loss. Alloreactive T cell receptor (TCR) clones were identified in pretransplant blood samples through mixed lymphocyte reactions, followed by single-cell RNA and TCR sequencing of the proliferated recipient T cells. Subsequently, these TCR clones were traced in the T RM cells of kidney explants, which were also subjected to single-cell RNA and TCR sequencing. The proportion of recipient-derived T RM cells expressing an alloreactive TCR in the 4 kidney explants varied from 0% to 9%. Notably, these alloreactive TCRs were predominantly found among CD4+ and CD8+ T RM cells with an effector phenotype. Intriguingly, these clones were present not only in recipient-derived T RM cells but also in donor-derived T RM cells, constituting up to 4% of the donor population, suggesting the presence of self-reactive T RM cells. Overall, our study demonstrates that T cells with alloreactive potential present in the peripheral blood prior to transplantation can infiltrate the kidney transplant and adopt a T RM phenotype.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.