Author Response: Quantity of SARS-CoV-2 RNA copies exhaled per minute during natural breathing over the course of COVID-19 infection
Rattachement africain : us, Afrique du Sud. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
SARS-CoV-2 is spread through exhaled breath of infected individuals. A fundamental question in understanding transmission of SARS-CoV-2 is how much virus an individual is exhaling into the environment while they breathe, over the course of their infection. Research on viral load dynamics during COVID-19 infection has focused on internal swab specimens, which provide a measure of viral loads inside the respiratory tract, but not on breath. Therefore, the dynamics of viral shedding on exhaled breath over the course of infection are poorly understood. Here, we collected exhaled breath specimens from COVID-19 patients and used RTq-PCR to show that numbers of exhaled SARS-CoV-2 RNA copies during COVID-19 infection do not decrease significantly until day 8 from symptom-onset. COVID-19-positive participants exhaled an average of 80 SARS-CoV-2 viral RNA copies per minute during the first 8 days of infection, with significant variability both between and within individuals, including spikes over 800 copies a minute in some patients. After day 8, there was a steep drop to levels nearing the limit of detection, persisting for up to 20 days. We further found that levels of exhaled viral RNA increased with self-rated symptom-severity, though individual variation was high. Levels of exhaled viral RNA did not differ across age, sex, time of day, vaccination status or viral variant. Our data provide a fine-grained, direct measure of the number of SARS-CoV-2 viral copies exhaled per minute during natural breathing—including 312 breath specimens collected multiple times daily over the course of infection—in order to fill an important gap in our understanding of the time course of exhaled viral loads in COVID-19.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Author Response: Quantity of SARS-CoV-2 RNA copies exhaled per minute during natural breathing over the course of COVID-19 infection
- Date Crossref
- 16/02/2024
- Éditeur
- eLife Sciences Publications, Ltd
- Type
- peer-review
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Northwestern University Institute for Global Health pays non établi dans la noticeUniversité ou école supérieure
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National Institute of Allergy and Infectious Diseases pays non établi dans la noticeStructure de recherche
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Vanqua Bio (United States) pays non établi dans la noticeEntreprise
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South African Medical Research Council Afrique du Sud (code pays fourni par la source)Institution
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Stellenbosch University Stellenbosch University, Afrique du Sud (code pays fourni par la source)Université ou école supérieure
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. 35 pays non établi dans la noticeInstitution
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Division of Microbiology and Infectious Diseases (DMID/NIAID/NIH) pays non établi dans la noticeInstitution
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Faculty of Medicine and Health Sciences Division of Molecular Biology and Human Genetics Stellenbosch University, Afrique du Sud (pays nommé en fin d’affiliation)Université ou école supérieure
Institute for Global Health — Northwestern University, National Institute of Allergy and Infectious Diseases et Vanqua Bio (United States), avec 5 autres affiliations. Pays d’affiliation : Afrique du Sud.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.