Comparison of milk microbiota between healthy and mastitic cows

Le résumé fourni par la source

Mammary gland infections occur due to bacterial changes in the mammary tissue. Studies conducted in recent years have reported variations in the most common bacteria differ according to geographical locations. California mastitis test (CMT), somatic cell count (SCC), and aerobic colony count (ACC) analyses were performed on approximately 50 mL of hygienically collected raw milk samples. Raw milk was also subjected to conventional bacteriological isolation and identification. Bacterial diversity and rates in raw milk were compared through metagenome analysis. Two samples, one from healthy milk and another from subclinical milk with mastitis, were independently tested to determine whether there were differences in the percentages (%) of bacterial phylum and genera detected as a result of metagenome analysis. As a result of the conventional isolation and identification of raw milk, EscherichiaShigella, Acinetobacter, Vibrio, Streptococcus, Pseudomonas, Lactococcus, Glutamicibacter and Bacillus genera, and Enterobacteriaceae family were frequently detected, respectively. As a result of metagenome analysis, the following phyla were detected in healthy raw milk: Firmicutes and Proteobacteria (7/7), Bacteroidota (6/7), and Actinobacteriota (4/7). In raw milk with subclinical mastitis, the detected phyla were Firmicutes and Proteobacteria (27/29), Actinobacteriota (11/29), and Bacteroidota (10/29). As a result of the statistical analysis, the frequency of Bacteriodata in healthy milk samples, as well as Enhydrobacter, Enterobacteriaceae, Paenibacillus, Macrococcus, Spingobacterium, and “Others”, were significantly higher than the incidence in milk samples with subclinical mastitis. The only exception was observed in Escherichia-Shigella genera, where the opposite situation was evident. As a result of metagenome studies conducted on the raw milk of animals with both healthy and subclinical mastitis, significant differences were detected in some phyla and genera. The findings of our study will shed light on mastitis treatment studies by improving the microbiota.

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