Compound Identification by HPLC-ESI-Q-TOF-MS/ MS Analysis of the Dichloromethane Fraction of Hyptis suaveolens Leaves After Extraction of the Essential Oil

Résumé fourni par la source

Hyptis suaveolens (Lamiaceae), widespread in tropical areas of America, Asia, and Africa, is used in more than twenty-two countries for its medicinal properties. The plant's leaves contain polyphenols, tannins, sterols and terpenes, saponins, flavonoids, quinones and anthraquinones before and after extraction of the essential oil. However, no molecular structures were identified in the leaves of the plant after extraction of the essential oil. So, after hydroethanolic (70/30; v/v) maceration of 100g of powder front and after extracting essential oil taken separately, a successively fractionate extract was obtained from solvents of increasing polarities. Next, the compounds of the dichloromethane fraction after extracting essential oil have been identified by HPLC-ESI- Q-TOF -MS/ MS method. As a result, the determination of the structures of nine (9) known compounds of the Hyptis genus was carried out by dereplication on the dichloromethane fraction from the hydroalcoholic extract (70%) of the plant's leaves after extraction of the essential oil. Of these nine (09) compounds, four (04) are phenolics and the remaining five (05) are terpenoids. Five (05) of these molecules had not yet been identified in Hyptis suaveolens leaves. This study shows that Hyptis suaveolens leaves after extraction of the essential oil are new source of bioactive compounds of pharmacological interest.

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