Activity of enzymes in the follicular fluid and outcome of in vitro fertilization
Rattachement africain : rs. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Defining certain parameters of the oocyte microenvironment that can be simply and quickly detected and that enable differentiation of oocytes that have better or worse quality could potentially increase the efficacy of the in vitro fertilization (IVF) method. Follicular fluid represents the microenvironment for the development of oocytes during different stages of maturation. Enzymes present in the follicular fluid may affect the quality of oocytes. There are inconsistencies in the literature concerning enzyme concentration in follicular fluid. The main aim of this study was to examine whether the concentration of enzymes aspartate aminotransferase, alanine aminotransferase (ALT), alkaline phosphatase, and lactate dehydrogenase in follicular fluid can influence the outcome of IVF. The study included 120 patients who were involved in the process of IVF. We used the follicular fluid obtained after follicular aspiration in the ovaries as biological material for the analysis. The values of enzymes LDL (method-kinetic UV test), aspartate aminotransferase (method-kinetic UV test), ALT (method-kinetic UV test) and alkaline phosphatase (method-colorimetric kinetic test) in the follicular fluid were determined on the analyzer АU 680, Becman Coulter. The criteria of the Istanbul consensus of clinical embryologists were used for the embryo assessment. The software package SPSS 20 was used for the statistical processing of data. The results of follicular fluid sample analysis showed a correlation between ALT concentration in the follicular fluid and IVF outcome. Based on our results, we can conclude that ALT concentration affects the outcome of IVF.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Activity of enzymes in the follicular fluid and outcome of in vitro fertilization
- Date Crossref
- 26/01/2024
- Éditeur
- Ovid Technologies (Wolters Kluwer Health)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Kragujevac pays non établi dans la noticeUniversité ou école supérieure
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Clinical Centre of Kragujevac pays non établi dans la noticeÉtablissement de santé
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University Clinical Center Kragujevac Department of Biomedically Assisted Fertilization pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Medical Sciences Department of Gynecology and Obstetrics pays non établi dans la noticeUniversité ou école supérieure
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103:4(e36851) pays non établi dans la noticeInstitution
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How to cite this article: Sorak MP pays non établi dans la noticeInstitution
University of Kragujevac, Clinical Centre of Kragujevac et Department of Biomedically Assisted Fertilization — University Clinical Center Kragujevac, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.