Novel diagnostic approach for amoebic liver abscess using cell free (cf) DNA: a prospective study
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Le résumé fourni par la source
Background Amoebic liver abscess (ALA) is commonly seen in tropical countries and diagnosis of ALA relies mainly on non-specific serological and imaging techniques as well as PCR from pus.Objective This study evaluated the potential of using cell free DNA (cfDNA) from serum and urine for diagnosing ALA.Methods We prospectively evaluated quantitative PCR (qPCR) for detection of cf DNA in serum and urine sample in all liver abscess patients. The samples were collected from patients reporting to emergency ward of Postgraduate Institute of Medical Education and Research, Chandigarh, India with symptoms suggestive of liver abscess. Real time PCR was done to detect cf DNA in serum and urine by targeting 99-bp unit of small subunit rRNA of Entamoeba histolytica and conventional PCR for pus.Results A total 113 samples (serum and urine) and 100 pus samples were analysed. A total of 62 ALA patients were confirmed; with maximum 57 patients detected by qPCR for cfDNA in the serum, 55 patients by PCR on pus aspirate and 50 ALA patients by qPCR for cfDNA in urine sample. Therefore, the sensitivity of qPCR for detection of cf DNA in serum was 91.94% and for urine was 80.65%.Conclusion A total of 11.2% of ALA patients were diagnosed only through detection of E. histolytica cf DNA in their serum and urine. Detection of cfDNA from serum, urine of ALA has a potential role in future especially for developing countries as it is a rapid, sensitive and patient friendly diagnostic approach.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Novel diagnostic approach for amoebic liver abscess using cell free (cf) DNA: a prospective study
- Date Crossref
- 19/12/2023
- Éditeur
- Informa UK Limited
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Post Graduate Institute of Medical Education and Research pays non établi dans la noticeUniversité ou école supérieure
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Department of Medical Parasitology pays non établi dans la noticeInstitution
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Department of Internal Medicine pays non établi dans la noticeInstitution
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Department of Radiodiagnosis & pays non établi dans la noticeInstitution
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Department of Hepatology pays non établi dans la noticeInstitution
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Department of Medical Microbiology pays non établi dans la noticeInstitution
Post Graduate Institute of Medical Education and Research, Department of Medical Parasitology et Department of Internal Medicine, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.