The potential link of COMP‐ADAMTS and Aggrecanase‐ADAMTS Genes with Osteoarthrit

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This project aimed to investigate the role of COMP‐ADAMTS and aggrecanase‐ADAMTS which are responsible for the destruction of cartilage in the ADAMTS ( a disintegrin and metalloproteinase with trombospondin motifs ) genes that are suspected to play role in the genetic etiology of the osteoarthritis (OA) which is the most common joint disease. 88 patients aged between 40–75 years, with the diagnosis of primary knee OA (according to American College of Rheumatology criteria for knee OA) and 88 healthy volunteers aged between 40–75 years, without OA enrolled in the study. Exclusion criteria include, previous knee surgery (total knee prosthesis etc.), inflammatory rheumatic diseases, secondary OA and malignancies. Radiologic staging of the participants is done, pain and functional status are assessed by visual analogue scale, WOMAC, Lequesne index. Blood samples obtained from the participants analyzed for ADAMTS gene family by ELISA and RT‐PCR. Blood samples obtained from the participants analyzed for ADAMTS ‐1,‐4,‐5,‐7,‐8,‐9,‐12,‐15,‐16 ve ‐18 genes by ELISA. Compared OD levels determined by ELISA reader of groups, OD level of ADAMTS‐4,‐8,‐9 and ‐18 was determined significantly high in the patient group compared to the control group. When examined the patients splitting (dividing) into two groups as early–advanced OA, results of ADAMTS‐4,‐5,‐8,‐9,‐15 ve ‐16 according to control group in early OA group, results of ADAMTS‐15 and ‐16 according to advanced OA group in early OA group and results of ADAMTS‐8, ‐9 and ‐18 according to control group in advanced OA group was seen significantly high. Statistical evaluation of RT‐PCR results showed that ADAMTS‐4,‐9 and ‐18 expressions are significantly decreased in the patient group compared to the control group, ADAMTS‐8 expression is not low enough to be meaningful statistically and there isn't any enough difference between two groups in ADAMTS‐5 expression level. Consequently; we observed that in the early stages ADAMTS‐4, ‐5, ‐15 and ‐16, in advanced stages ADAMTS may be the main aggrecanase responsible for the destruction of cartilage in human knee OA. ADAMTS‐8 and ‐9 were determined to play a role in both early and advanced stages of cartilage destruction in OA. We have seen that ADAMTS‐1, ‐7 and ‐12 were not playing an important role in the pathogenesis of OA. Support or Funding Information Tubitak and Turgut Ozal University Hospital Project Management Ofice

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