Fluid and Bubble Flow Detach Adherent Cancer Cells to Form Spheroids on a Random Positioning Machine
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Le résumé fourni par la source
In preparing space and microgravity experiments, the utilization of ground-based facilities is common for initial experiments and feasibility studies. One approach to simulating microgravity conditions on Earth is to employ a random positioning machine (RPM) as a rotary bioreactor. Combined with a suitable low-mass model system, such as cell cultures, these devices simulating microgravity have been shown to produce results similar to those obtained in a space experiment under real microgravity conditions. One of these effects observed under real and simulated microgravity is the formation of spheroids from 2D adherent cancer cell cultures. Since real microgravity cannot be generated in a laboratory on Earth, we aimed to determine which forces lead to the detachment of individual FTC-133 thyroid cancer cells and the formation of tumor spheroids during culture with exposure to random positioning modes. To this end, we subdivided the RPM motion into different static and dynamic orientations of cell culture flasks. We focused on the molecular activation of the mechanosignaling pathways previously associated with spheroid formation in microgravity. Our results suggest that RPM-induced spheroid formation is a two-step process. First, the cells need to be detached, induced by the cell culture flask's rotation and the subsequent fluid flow, as well as the presence of air bubbles. Once the cells are detached and in suspension, random positioning prevents sedimentation, allowing 3D aggregates to form. In a comparative shear stress experiment using defined fluid flow paradigms, transcriptional responses were triggered comparable to exposure of FTC-133 cells to the RPM. In summary, the RPM serves as a simulator of microgravity by randomizing the impact of Earth's gravity vector especially for suspension (i.e., detached) cells. Simultaneously, it simulates physiological shear forces on the adherent cell layer. The RPM thus offers a unique combination of environmental conditions for in vitro cancer research.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Fluid and Bubble Flow Detach Adherent Cancer Cells to Form Spheroids on a Random Positioning Machine
- Date Crossref
- 20/11/2023
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Otto-von-Guericke-Universität Magdeburg pays non établi dans la noticeUniversité ou école supérieure
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University Hospital Magdeburg Institute of Anatomy pays non établi dans la noticeÉtablissement de santé
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University of South Australia UniSA Clinical and Health Sciences pays non établi dans la noticeUniversité ou école supérieure
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Institute of Molecular Bioimaging and Physiology pays non établi dans la noticeStructure de recherche
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National Research Council pays non établi dans la noticeOrganisation à but non lucratif
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Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR) pays non établi dans la noticeStructure de recherche
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Lucerne University of Applied Sciences and Arts Institute of Medical Engineering pays non établi dans la noticeUniversité ou école supérieure
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Aarhus University Department of Biomedicine pays non établi dans la noticeUniversité ou école supérieure
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Otto-von-Guericke University Department of Microgravity and Translational Regenerative Medicine pays non établi dans la noticeUniversité ou école supérieure
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Institute of Bioimaging and Molecular Physiology pays non établi dans la noticeStructure de recherche
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Institute of Aerospace Medicine Department of Gravitational Biology pays non établi dans la noticeStructure de recherche
Otto-von-Guericke-Universität Magdeburg, Institute of Anatomy — University Hospital Magdeburg et UniSA Clinical and Health Sciences — University of South Australia, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.