BIOM-33. TRNA FRAGMENTS IN PLASMA EXTRACELLULAR VESICLES FROM HIGH GRADE GLIOMA PATIENTS, POTENTIAL BIOMARKERS?

Résumé fourni par la source

Abstract Transfer RNA (tRNA) are non-coding RNAs responsible for bringing the amino acids to the ribosome during translation. Recent studies in cancer have shown that fragments (tRFs) derived from tRNA cleavage serve regulatory roles in epigenetics, signalling and translation. tRFs are also released from cells bound to proteins and can be found in extracellular vesicles to perform some aspects of paracrine signalling. Utilizing high throughput small RNA sequencing we have identified over 750 uniquely or multi-mapped (6.9% of total sRNA mapped reads) tRNA and tRFs in extracellular vesicles of high-grade glioma patient plasma (IDH-WT). Consistent with novel literature in other cancers there was a general trend to decreased expression of tRNA and tRFs (n = 76 with FDR < 0.05) in high grade glioma plasma EVs compared to controls. However, further analysis of tRFs reveals a much more complex story, as fragment distribution differs between cancer and controls. tRNAs and tRFs may serve as important biomarkers and regulators with therapeutic potential in the future.

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