OSMAC Approach and Cocultivation for the Induction of Secondary Metabolism of the Fungus Pleotrichocladium opacum

Le résumé fourni par la source

High Resolution Image Download MS PowerPoint Slide The application of an OSMAC (One Strain–Many Compounds) approach on the fungus Pleotrichocladium opacum, isolated from a soil sample collected on the coast of Asturias (Spain), using different culture media, chemical elicitors, and cocultivation techniques resulted in the isolation and identification of nine new compounds ( 8, 9, 12, 15 – 18, 20, 21 ), along with 15 known ones ( 1 – 7, 10, 11, 14, 19, 22 – 25 ). Compounds 1 – 9 were detected in fungal extracts from JSA liquid fermentation, compounds 10 – 12 were isolated from a solid rice medium, whereas compounds 14 and 15 were isolated from a solid wheat medium. Addition of 5-azacytidine to the solid rice medium caused the accumulation of compounds 16 – 18, whereas adding N -acetyl- d -glucosamine triggered the production of two additional metabolites, 19 and 20 . Finally, cocultivation of the fungus Pleotrichocladium opacum with Echinocatena sp. in a solid PDA medium led to the production of five additional natural products, 21 – 25 . The structures of the new compounds were elucidated by HRESIMS and 1D and 2D NMR as well as by comparison with literature data. DP4+ and mix- J -DP4 computational methods were applied to determine the relative configurations of the novel compounds, and in some cases, the absolute configurations were assigned by a comparison of the optical rotations with those of related natural products.

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