EVALUATION OF THE ROLE OF MAST CELLS IN GALLBLADDER DYSPLASIA

Le résumé fourni par la source

Aim: Although the gallbladder is a frequently evaluated material, our knowledge in this area is limited, except for our predictions about some histological features in gallbladder dysplasia. In addition, cellular behaviors and expression profiles associated with Mast Cells (MCs) in gallbladder dysplasia remain unclear. Material-Method: In our study, gallbladder tissues with mild and severe dysplasia obtained by open cholecystectomy and laparoscopy were compared with control tissues and examined in terms of MCs markers Fc epsilon (ε) RI Alpha (FcεRIα) and FcεRI Gamma (FcεRIγ), Anti-Mast Chemase (McC) and Mast Cell Tryptase (McT). The status of these specific markers, associated with inflammatory processes and whose behavior in dysplasia cannot be entirely determined, were evaluated in tissues immunohistochemically and terms of general tissue structure with HE and Masson Trichrome staining. The tissue protein expression level was measured with Image J, and the difference between the groups was compared with One-way ANOVA, and p<0.05 was accepted as a significant difference. Results: Our results show that FcεRIα, FcεRIγ, McT, and McC levels increase in dysplasia compared to the control. However, there is a decrease as the severity of dysplasia increases, especially McC and McT, suggesting that MC activity is impaired (p<0.05). Conclusion: Finally, there seems to be a relationship between dysplasias and MCs, and it is thought that it should be investigated in more detail.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.