Efficient qPCR estimation and discrimination of airborne inoculum of Leptosphaeria maculans and L. biglobosa , the causal organisms of phoma leaf spotting and stem canker of oilseed rape
Rattachement africain : pl, gb. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: Detection of the inoculum of phytopathogens greatly assists in the management of diseases, but is difficult for pathogens with airborne fungal propagules. Here, we present experiments to determine the abundance and distribution frequencies of the ascospores of Leptosphaeria (Plenodomus) species that were collected on the tapes of volumetric Hirst-type traps near oilseed rape fields in Poznan, Poland and Harpenden, UK. Fungal detection and species discrimination were achieved using a SYBR-Green quantitative polymerase chain reaction (qPCR) with two different pairs of primers previously reported to differentiate Leptosphaeria maculans (Plenodomus lingam) or L. biglobosa (P. biglobosus). RESULTS: of air. The primer pairs differed in the correlation coefficients obtained between DNA yields and the daily abundance of ascospores that were quantified by microscopy on duplicate halves of the spore trap tapes. Important differences in the specificity and sensitivity of the published SYBR-Green assays were also found, indicating that the Liu primers did not detect L. biglobosa subclade 'canadensis', whereas the Mahuku primers detected L. biglobosa subclade 'canadensis' and also the closely related Plenodomus dezfulensis. CONCLUSIONS: Comparisons confirmed that application of qPCR assays to spore trap samples can be used for the early detection, discrimination and quantification of aerially dispersed L. maculans and L. biglobosa propagules before leaf spot symptoms are visible in winter oilseed rape fields. The specificity of the primers must be taken into consideration because the final result will greatly depend on the local population of the pathogen. © 2023 Society of Chemical Industry.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Efficient <scp>qPCR</scp> estimation and discrimination of airborne inoculum of <scp><i>Leptosphaeria maculans</i></scp> and <scp><i>L. biglobosa</i></scp>, the causal organisms of phoma leaf spotting and stem canker of oilseed rape
- Date Crossref
- 06/12/2023
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Institute of Plant Genetics pays non établi dans la noticeStructure de recherche
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Polish Academy of Sciences pays non établi dans la noticeOrganisme public
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Rothamsted Research pays non établi dans la noticeStructure de recherche
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University of Hertfordshire Food and Environmental Management Research pays non établi dans la noticeUniversité ou école supérieure
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Joanna Kaczmarek and Jonathan S West are to be regarded as joint first authors of this paper pays non établi dans la noticeInstitution
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School of Life and Medical Sciences Food and Environmental Management Research pays non établi dans la noticeUniversité ou école supérieure
Institute of Plant Genetics, Polish Academy of Sciences et Rothamsted Research, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.