Detection and isolation of infectious SARS-CoV-2 omicron subvariants collected from residential settings
Rattachement africain : us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Airborne transmission of infectious (viable) SARS-CoV-2 is increasingly accepted as the primary manner by which the virus is spread from person to person. Risk of exposure to airborne virus is higher in enclosed and poorly ventilated spaces. We present a study focused on air sampling within residences occupied by individuals with COVID-19. Air samplers (BioSpot-VIVAS, VIVAS, and BC-251) were positioned in primary- and secondary-occupancy regions in seven homes. Swab samples were collected from high-touch surfaces. Isolation of SARS-CoV-2 was attempted for samples with virus detectable by RT-qPCR. Viable virus was quantified by plaque assay, and complete virus genome sequences were obtained for selected samples from each sampling day. SARS-CoV-2 was detected in 24 of 125 samples (19.2%) by RT-qPCR and isolated from 14 (11.2%) in cell cultures. It was detected in 80.9% (17/21) and cultured from 61.9% (13/21) of air samples collected using water condensation samplers, compared to swab samples which had a RT-qPCR detection rate of 10.5% (4/38) and virus isolation rate of 2.63% (1/38). No statistically significant differences existed in the likelihood of virus detection by RT-qPCR or amount of infectious virus in the air between areas of primary and secondary occupancy within residences. Our work provides information about the presence of SARS-CoV-2 in the air within homes of individuals with COVID-19. Information herein can help individuals make informed decisions about personal exposure risks when sharing indoor spaces with infected individuals isolating at home and further inform health departments and the public about SARS-CoV-2 exposure risks within residences.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Detection and isolation of infectious SARS-CoV-2 omicron subvariants collected from residential settings
- Date Crossref
- 07/09/2023
- Éditeur
- Informa UK Limited
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Florida Department of Environmental Engineering Sciences pays non établi dans la noticeUniversité ou école supérieure
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University of Florida Health pays non établi dans la noticeUniversité ou école supérieure
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University of Georgia Department of Statistics pays non établi dans la noticeUniversité ou école supérieure
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Aerosol Dynamics (United States) pays non établi dans la noticeEntreprise
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University of Miami Department of Chemical pays non établi dans la noticeUniversité ou école supérieure
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Aerosol Dynamics Inc pays non établi dans la noticeEntreprise
Department of Environmental Engineering Sciences — University of Florida, University of Florida Health et Department of Statistics — University of Georgia, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.