Biosynthesis of Functional Silver Nanoparticles Using Callus and Hairy Root Cultures of Aristolochia manshuriensis
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Le résumé fourni par la source
This study delves into the novel utilization of Aristolochia manshuriensis cultured cells for extracellular silver nanoparticles (AgNPs) synthesis without the need for additional substances. The presence of elemental silver has been verified using energy-dispersive X-ray spectroscopy, while distinct surface plasmon resonance peaks were revealed by UV-Vis spectra. Transmission and scanning electron microscopy indicated that the AgNPs, ranging in size from 10 to 40 nm, exhibited a spherical morphology. Fourier-transform infrared analysis validated the abilty of A. manshuriensis extract components to serve as both reducing and capping agents for metal ions. In the context of cytotoxicity on embryonic fibroblast (NIH 3T3) and mouse neuroblastoma (N2A) cells, AgNPs demonstrated varying effects. Specifically, nanoparticles derived from callus cultures exhibited an IC50 of 2.8 µg/mL, effectively inhibiting N2A growth, whereas AgNPs sourced from hairy roots only achieved this only at concentrations of 50 µg/mL and above. Notably, all studied AgNPs’ treatment-induced cytotoxicity in fibroblast cells, yielding IC50 values ranging from 7.2 to 36.3 µg/mL. Furthermore, the findings unveiled the efficacy of the synthesized AgNPs against pathogenic microorganisms impacting both plants and animals, including Agrobacterium rhizogenes, A. tumefaciens, Bacillus subtilis, and Escherichia coli. These findings underscore the effectiveness of biotechnological methodologies in offering advanced and enhanced green nanotechnology alternatives for generating nanoparticles with applications in combating cancer and infectious disorders.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Biosynthesis of Functional Silver Nanoparticles Using Callus and Hairy Root Cultures of Aristolochia manshuriensis
- Date Crossref
- 01/09/2023
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Federal Scientific Center of the East Asia Terrestrial Biodiversity FEB RAS pays non établi dans la noticeStructure de recherche
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Far Eastern Federal University Institute of Life Sciences and Biomedicine pays non établi dans la noticeUniversité ou école supérieure
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Institute of Chemistry pays non établi dans la noticeStructure de recherche
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Central Research Institute of Epidemiology Department of Molecular Diagnostics and Epidemiology pays non établi dans la noticeStructure de recherche
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Geological Institute pays non établi dans la noticeStructure de recherche
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Far Eastern Branch of the Russian Academy of Sciences A.V. Zhirmunsky National Scientific Center of Marine Biology pays non établi dans la noticeStructure de recherche
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A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch of the Russian Academy of Sciences pays non établi dans la noticeStructure de recherche
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Far Eastern Branch of Russian Academy of Sciences Federal Scientific Center of the East Asia Terrestrial Biodiversity pays non établi dans la noticeStructure de recherche
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School of Advanced Engineering Studies “Institute of Biotechnology pays non établi dans la noticeUniversité ou école supérieure
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School of Advanced Engineering Studies "Institute of Biotechnology pays non établi dans la noticeUniversité ou école supérieure
Federal Scientific Center of the East Asia Terrestrial Biodiversity FEB RAS, Institute of Life Sciences and Biomedicine — Far Eastern Federal University et Institute of Chemistry, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.