Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology
Rattachement africain : it. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Estimation of age represents a central focus in the veterinary forensic pathology field. Currently, the visual examination of the dentition and the skeletal age are the main methods to estimate the age of puppies. Nevertheless, these methods are affected by a broad range of variables. In contrast, the kidney is characterized by a specific postnatal development. In human glomerulogenesis, fetal mesangial cells change their immunohistochemical phenotypes with maturation. Therefore, we hypothesized that histological and immunohistochemical examinations of the kidney can be used together as an indirect parameter for age determination in puppies' cadavers. Forty-five puppies' cadavers were divided into five groups defined by age (Group A= 0-15 days, Group B = 16-45 days, Group C = 46-85 days, Group D = 86-105 days, Group E= 105-365 days). For each case, kidney samples were collected and processed for histopathological (for morphometrical study of the glomerulus) and immunohistochemical (for the immunolocalization of the α-SMA protein) studies. Morphometrical study allowed us to observe statistical differences in the mean glomerulus numbers per field among assessed groups. Similarly, immunohistochemical examination showed differences in SMA expression among groups. Our findings suggest a potential use of kidney morphometrical and immunohistochemical examinations together as an indirect parameter to assess the age of illegally imported puppies.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology
- Date Crossref
- 18/08/2023
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
University of Naples Federico II Unit of Pathology pays non établi dans la noticeUniversité ou école supérieure
-
Istituto Zooprofilattico Sperimentale del Mezzogiorno pays non établi dans la noticeStructure de recherche
-
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana pays non établi dans la noticeStructure de recherche
-
University of Salerno Department of Pharmacy pays non établi dans la noticeUniversité ou école supérieure
-
Unit of Forensic Veterinary Medicine pays non établi dans la noticeInstitution
-
National Reference Centre for Veterinary Forensic Medicine pays non établi dans la noticeInstitution
Unit of Pathology — University of Naples Federico II, Istituto Zooprofilattico Sperimentale del Mezzogiorno et Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.