De novo assembly and annotation of the Amblyomma hebraeum tick midgut transcriptome response to Ehrlichia ruminantium infection
Rattachement africain : Kenya, de, Afrique du Sud. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
The South African bont tick Amblyomma hebraeum is a hematophagous vector for the heartwater disease pathogen Ehrlichia ruminantium in southern Africa. During feeding, the tick's enterocytes express proteins that perform vital functions in blood digestion, including proteins that may be involved in E. ruminantium acquisition, colonization or immunity. To delineate the molecular mechanism of midgut response to E. ruminantium infection, we performed comparative analyses of midgut transcriptomes of E. ruminantium infected engorged A. hebraeum nymphs, and infected adult male and female ticks with their corresponding matched uninfected controls, before and during feeding. A total of 102,036 unigenes were annotated in public databases and their expression levels analyzed for engorged nymphs as well as unfed and partly-fed adult ticks. There were 2,025 differentially expressed genes (DEGs) in midguts, of which 1,225 unigenes were up-regulated and 800 unigenes were down-regulated in the midguts of infected ticks. Annotation of DEGs revealed an increase in metabolic and cellular processes among E. ruminantium infected ticks. Notably, among the infected ticks, there was up-regulation in the expression of genes involved in tick immunity, histone proteins and oxidative stress responses. We also observed up-regulation of glycoproteins that E. ruminantium could potentially use as docking sites for host cell entry. Insights uncovered in this study offer a platform for further investigations into the molecular interaction between E. ruminantium and A. hebraeum.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- De novo assembly and annotation of the Amblyomma hebraeum tick midgut transcriptome response to Ehrlichia ruminantium infection
- Date Crossref
- 14/08/2023
- Éditeur
- Public Library of Science (PLoS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Egerton University Department of Biochemistry and Molecular Biology Egerton University, Kenya (code pays fourni par la source)Université ou école supérieure
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Freie Universität Berlin pays non établi dans la noticeUniversité ou école supérieure
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University of Pretoria University of Pretoria, Afrique du Sud (code pays fourni par la source)Université ou école supérieure
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Kisii University Kenya (code pays fourni par la source)Université ou école supérieure
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Institute of Parasitology and Tropical Veterinary Medicine pays non établi dans la noticeStructure de recherche
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Veterinary Centre for Resistance Research (TZR) pays non établi dans la noticeInstitution
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Faculty of Veterinary Science Department of Veterinary Tropical Diseases University of Pretoria, Afrique du Sud (pays nommé en fin d’affiliation)Université ou école supérieure
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School of Health Sciences Department of Medical Biochemistry Kenya (pays nommé en fin d’affiliation)Université ou école supérieure
Department of Biochemistry and Molecular Biology — Egerton University (Egerton University, Kenya), Freie Universität Berlin et University of Pretoria (University of Pretoria, Afrique du Sud), avec 5 autres affiliations. Pays d’affiliation : Kenya, Afrique du Sud.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.