Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis
Rattachement africain : cn, Éthiopie. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: In the normal life cycle of the parasite (Echinococcus multilocularis) that causes alveolar echinococcosis, domestic and wild carnivores act as definitive hosts, and rodents act as intermediate hosts. The presented study contributes to the research on the distribution and transmission pattern of E. multilocularis in China having identified sheep as an unusual intermediate host taking part in the domestic transmission of alveolar echinococcosis in Gansu Province, China. METHODS: From 2020 to 2021, nine whitish different cyst-like were collected from the liver of sheep in Gansu Province for examination. A near complete mitochondrial (mt) genome and selected nuclear genes were amplified from the cyst-like lesion for identification. To confirm the status of the specimen, comparative analysis with reference sequences, phylogenetic analysis, and network analysis were performed. RESULTS: The isolates displayed ≥ 98.87% similarity to E. multilocularis NADH dehydrogenase sub-unit 1 (nad1) (894 bp) reference sequences deposited in GenBank. Furthermore, amplification of the nad4 and nad2 genes also confirmed all nine samples as E. multilocularis with > 99.30% similarity. Additionally, three nuclear genes, pepck (1545 bp), elp-exons VII and VIII (566 bp), and elp-exon IX (256 bp), were successfully amplified and sequenced for one of the isolates with 98.42% similarity, confirming the isolates were correctly identified as E. multilocularis. Network analysis also correctly placed the isolates with other E. multilocularis. CONCLUSIONS: As a result of the discovery of E. multilocularis in an unusual intermediate host, which is considered to have the highest zoonotic potential, the result clearly demonstrated the necessity for expanded surveillance in the area.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis
- Date Crossref
- 10/08/2023
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Lanzhou Veterinary Research Institute pays non établi dans la noticeStructure de recherche
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Chinese Academy of Agricultural Sciences pays non établi dans la noticeOrganisme public
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Mekelle University Department of Veterinary Clinical Medicine and Epidemiology Mekelle University, Éthiopie (code pays fourni par la source)Université ou école supérieure
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Lanzhou University pays non établi dans la noticeUniversité ou école supérieure
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Gansu Provincial Center for Disease Control and Prevention pays non établi dans la noticeOrganisme public
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Jingning County People's Hospital pays non établi dans la noticeÉtablissement de santé
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Addis Ababa University Aklilu Lemma Institute of Pathobiology Addis Ababa University, Éthiopie (code pays fourni par la source)Université ou école supérieure
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College of Veterinary Medicine State Key Laboratory for Animal Disease Control and Prevention pays non établi dans la noticeUniversité ou école supérieure
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Gansu Animal Centre for Disease Control and Prevention pays non établi dans la noticeInstitution
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Jingyuan County Animal Centre for Disease Control and Prevention pays non établi dans la noticeInstitution
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Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses pays non établi dans la noticeInstitution
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China. jiawanzhong@caas.cn Yangzhou pays non établi dans la noticeOrganisation à but non lucratif
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences et Department of Veterinary Clinical Medicine and Epidemiology — Mekelle University (Mekelle University, Éthiopie), avec 9 autres affiliations. Pays d’affiliation : Éthiopie.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.