The role of specific isoforms of CaV2 and the common C-terminal of CaV2 in calcium channel function in sensory neurons of Aplysia

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Abstract Habituation is the diminishing of a physiological response to a frequently repeated stimulus. In Aplysia, habituation to touch is mediated by the depression of the response to sensory neuron firing mediated by a decrease in transmitter release even after modest rates of action potential firing. This depression is not common among Aplysia synaptic connections suggesting the presence of a release apparatus specialized for this depression. This depression can be reversed through activation of protein kinase C and this is thought to underlie the behavior of dishabituation. We have previously noted that the calcium channel required for release, ApCaV2, has many splice isoforms in Aplysia. We found that specific splice forms of ApCaV2 and splice forms for interacting partners of ApCaV2 are preferentially used in sensory neurons, consistent with a specialized release apparatus. However, we were not able to find a specific splice uniquely required for synaptic depression. The C-terminus of ApCaV2 alpha1 subunit retains conserved binding sites for Aplysia rab-3 interacting molecule (ApRIM) and ApRIM-binding protein (ApRBP) and the C-terminus is required for full synaptic expression of ApCaV2.

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