Promising investigative study: valorizing sardine scales by-products as eco-friendly carbonated calcium phosphate biomaterials for bone tissue engineering

Le résumé fourni par la source

The present work studied the physicochemical and biological properties of fish scales composed of natural carbonated hydroxyapatite, called SS-CaPx (where x represents sample indexation). Fish scales and mainly European sardine ( Sardina pilchardus) scales were used, their behavior was compared with those of healthy rat bone (HRB) and synthetic hydroxyapatite (HA syn). A simulation of the crystallite size on the X-ray patterns confirmed the nanocrystals present in the natural matrix (SS-CaP1 and HRB) produce broad peaks compared to HA syn. The broad peaks observed in SS-CaP1 are primarily due to simultaneous elastic and inelastic scattering, rather than low crystallinity. The analysis shows that the SS-CaP1 is composed of approximately 10 nm in length and 3 nm in width sized nanocrystal, whereas HA syn is composed of particles of about 34 nm in length and 12 nm in width. Calcium phosphate molar ratio (Ca/P) of SS-CaP1 does not exceed 1.84. Cytotoxicity assay (MTT) shows significant increases in the proliferation and activity of osteoblasts, fibroblasts and mesenchymal stem cells over the chosen sample (SS-CaP1) after 72 hours of culture. These results confirm the biomaterials derived from sardine scales SS-CaPx have biologically the potential for use as bone regenerative materials .

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.