Single Nucleotide Polymorphism in Glutathione Synthetase Gene in Indian Population with Type 2 Diabetes

Résumé fourni par la source

Introduction: Our earlier study has confirmed the presence of significantly low glutathione (GSH) in individuals with type 2 diabetes. Low levels of GSH in addition to other factors can be due to the presence of single nucleotide polymorphism (SNP) in the gene glutathione synthetase (GSS). We have therefore checked the presence of SNPs in GSS, known to be associated with GSH deficiency, in the Indian population. Methods: Blood samples were collected from cases (people with diabetes) and controls (individuals without diabetes) for GSH estimation and DNA isolation. Amplicons obtained against selected exons of GSS after polymerase chain reaction amplification were sequenced and analyzed for the presence of SNPs. Results: A significantly low GSH was found in the case compared to control group ( P < 0.001). However, we did not find any reported or novel SNPs associated with GSH deficiency in either of the groups. Conclusion: Data obtained suggest that the lower GSH found in individuals with diabetes of Indian origin is not contributed by SNPs in GSS reported otherwise in different population.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.