Fabrication of Aptasensors Modified by MWCNTs-CS / Fe3O4- CS Based on SPEs

Résumé fourni par la source

In this paper, A new electrochemical aptamer biosensor for detecting tetracycline (TET) has been developed based on the signal amplification of MWCNTs-CS and Fe3O4-CS. The multiwalled carbon nanotube (MWCNTs) and ferroferric oxide (Fe3O4) were dispersed in chitosan evenly. Then the MWCNTs-CS , Fe3O4-CS and the aptamer of anti-TET were modified on the surface of screen printing electrode respectively for preparation of the aptasensor. The electrochemistry properties of the surface modification were investigated by using cyclic voltammetry (CV). The proposed aptasensor showed a high sensitivity and a good stability. The proposed aptasensor exhibited a wide linear range from 10-2 to 10-9 M and with the correlation coefficients of 0.9651. The detection limit for tetracycline was 10-9 M. The application of this method for samples of TET-spiked raw milk suggested satisfactory recoveries between 92% and 98%. The proposed method was proven to be a feasible quantitative method for tetracycline analysis.

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