CRISPR Screens in Colon Cancer

Le résumé fourni par la source

CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats – Cas9) is an innovative technology that enables researchers to interrogate the function of genes through precise editing of genes. Researchers have recently utilized this technology to assess the function of all genes in a system using CRISPR-Cas9 screens. These screens are powerful techniques that enable the unbiased interrogation of gene function in various model systems and organisms. These pooled screens employ a predefined set of genetic perturbations called sgRNAs, which are introduced into a pool of cells. This pool of cells, each with its own genetic perturbation, is then grown in competition with each other. After a period of competition, the effects of each perturbation are assessed through the sequencebased counting of each specific mutation, or sgRNA. CRISPR-Cas9 screens have revealed numerous molecular pathways that may confer resistance or sensitivity to various biological challenges. This review will describe how these techniques have advanced our understanding of colon cancer and demonstrate how these screens can be modeled and optimized in silico using computational simulation tools. It will further describe how these screens have been applied to living organisms and provide insight into how these may advance our understanding of various biological mechanisms and organ functions.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.