Study on the identification and quantification of sodium benzoate in different brands of jelly by high performance liquid chromatography

Le résumé fourni par la source

A reversed-phase high-performance liquid chromatography analysis was carried out for rapid determination and quantification of sodium benzoate in different jelly products. Sodium benzoate is allowed food additive by universal laws in processing in restrictive amounts, but its content must be declared and must not exceed the established limits by legislation. An experimental study for the level of sodium benzoate in different brands of jelly available in the markets, stores and shops in the Tangail region in Bangladesh was determined by high-performance liquid chromatography. Chromatographic separation was achieved with isocratic solvent system of sodium acetate and acetic acid buffer (pH = 4.0): acetonitrile as 70:30 with a flow rate of 0.8 mL/min; chromatograms were recorded at 254 nm. The injection volume was 20 µL. The concentrations of sodium benzoate in all the samples were calculated by the external standard method with calibration of correlation coefficient 0.985 from the standards calibration curves. The limit of detection and quantification for sodium benzoate was 0.0003 mg/100 mL and 0.0009 mg/100 mL respectively. Quantification of the selected brand jellies revealed that the level of the used sodium benzoate was within the FDA standard range. But by comparing with the Bangladesh Standard and Testing Institute (BSTI), Brand-3 jelly samples were found to exceed the current legal limits.

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