Biomechanical and Biological Characterization of XGel, a Human‐Derived Hydrogel for Stem Cell Expansion and Tissue Engineering
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Le résumé fourni par la source
Hydrogels are 3D scaffolds used as alternatives to in vivo models for disease modeling and delivery of cells and drugs. Existing hydrogel classifications include synthetic, recombinant, chemically defined, plant- or animal-based, and tissue-derived matrices. There is a need for materials that can support both human tissue modeling and clinically relevant applications requiring stiffness tunability. Human-derived hydrogels are not only clinically relevant, but they also minimize the use of animal models for pre-clinical studies. This study aims to characterize XGel, a new human-derived hydrogel as an alternative to current murine-derived and synthetic recombinant hydrogels that features unique physiochemical, biochemical, and biological properties that support adipocyte and bone differentiation. Rheology studies determine the viscosity, stiffness, and gelation features of XGel. Quantitative studies for quality control support consistency in the protein content between lots. Proteomics studies reveal that XGel is predominantly composed of extracellular matrix proteins, including fibrillin, collagens I-VI, and fibronectin. Electron microscopy of the hydrogel provides phenotypic characteristics in terms of porosity and fiber size. The hydrogel demonstrates biocompatibility as a coating material and as a 3D scaffold for the growth of multiple cell types. The results provide insight into the biological compatibility of this human-derived hydrogel for tissue engineering.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Biomechanical and Biological Characterization of XGel, a Human‐Derived Hydrogel for Stem Cell Expansion and Tissue Engineering
- Date Crossref
- 26/05/2023
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Louisiana State University Agricultural Center pays non établi dans la noticeUniversité ou école supérieure
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LSU New Orleans pays non établi dans la noticeUniversité ou école supérieure
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University of Karachi pays non établi dans la noticeUniversité ou école supérieure
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International Center for Chemical and Biological Sciences pays non établi dans la noticeStructure de recherche
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The Ohio State University pays non établi dans la noticeUniversité ou école supérieure
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Department of Biological and Agricultural Engineering Louisiana State University and Agricultural Center Baton Rouge LA 70803 USA pays non établi dans la noticeUniversité ou école supérieure
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Obatala Sciences Inc. New Orleans LA 70148 USA pays non établi dans la noticeEntreprise
Louisiana State University Agricultural Center, LSU New Orleans et University of Karachi, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.