Establishment of neural stem cells from fetal monkey brain for neurotoxicity testing
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Le résumé fourni par la source
Neurotoxicity assessments are generally performed using laboratory animals. However, as in vitro neurotoxicity models are continuously refined to reach adequate predicative concordance with in vivo responses, they are increasingly used for some endpoints of neurotoxicity. In this study, gestational day 80 fetal rhesus monkey brain tissue was obtained for neural stem cells (NSCs) isolation. Cells from the entire hippocampus were harvested, mechanically dissociated, and cultured for proliferation and differentiation. Immunocytochemical staining and biological assays demonstrated that the harvested hippocampal cells exhibited typical NSC phenotypes in vitro: (1) cells proliferated vigorously and expressed NSC markers nestin and sex-determining region Y-box 2 (SOX2) and (2) cells differentiated into neurons, astrocytes, and oligodendrocytes, as confirmed by positive staining with class III β-tubulin, glial fibrillary acidic protein, and galactocerebroside, respectively. The NSC produced detectable responses following neurotoxicant exposures (e.g. trimethyltin and 3-nitropropionic acid). Our results indicated that non-human primate NSCs may be a practical tool to study the biology of neural cells and to evaluate the neurotoxicity of chemicals in vitro, thereby providing data that are translatable to humans and may also reduce the number of animals needed for developmental neurotoxicological studies.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Establishment of neural stem cells from fetal monkey brain for neurotoxicity testing
- Date Crossref
- 01/04/2023
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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National Center for Toxicological Research pays non établi dans la noticeStructure de recherche
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United States Food and Drug Administration pays non établi dans la noticeOrganisme public
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Center for Drug Evaluation and Research pays non établi dans la noticeStructure de recherche
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Division of Neurotoxicology pays non établi dans la noticeInstitution
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Division of Genetic and Molecular Toxicology pays non établi dans la noticeInstitution
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Office of Pharmaceutical Quality pays non établi dans la noticeInstitution
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Office of Director pays non établi dans la noticeInstitution
National Center for Toxicological Research, United States Food and Drug Administration et Center for Drug Evaluation and Research, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.