Accès ouvert déclaré 2023 article

Evaluation of Autoantibody Binding to Cardiac Tissue in Multisystem Inflammatory Syndrome in Children and COVID-19 Vaccination–Induced Myocarditis

Harsita Patel, Amalia Sintou, Rasheda A. Chowdhury, STEPHEN M. ROTHERY, Alma Iacob, Sanjay Kumar Prasad, Peter P. Rainer, Federico Martinón‐Torres, Vanessa Sancho‐Shimizu, Chisato Shimizu, Kirsten Dummer, Adriana H. Tremoulet, Jane C. Burns, Susanne Sattler, Michael L. Levin, Aubrey J. Cunnington, Jethro Adam Herberg, Myrsini Kaforou, Victoria J. Wright, Evangelos Bellos, Claire M. Broderick, Samuel Channon‐Wells, Samantha Cooray, Tisham De, Giselle D’Souza, Leire Estramiana Elorrieta, Diego Estrada‐Rivadeneyra, Rachel Galassini, Dominic Habgood-Coote, Shea Hamilton, Heather Jackson, James C. Kavanagh, Ilana Keren, Mahdi Moradi Marjaneh, Stephanie Menikou, Samuel Nichols, Ruud Nijman, Ivana Pennisi, Oliver Powell, Ruth Reid, Priyen Shah, Ortensia Vito, Elizabeth A. Whittaker, Clare A. Wilson, Rebecca Womersley, Amina Abdulla, Sarah Darnell, Sobia Mustafa, Pantelis Georgiou, Jesus-Rodriguez Manzano, Nicolas Moser, Michael J. Carter, Shane M. Tibby, Jonathan B. Cohen, Francesca J. Davis, Julia M. Kenny, Paul Wellman, Marie C. White, Matthew Fish, A.R. Jennings, Manu Shankar‐Hari, Katy Fidler, Dan Agranoff, Vivien Richmond, Mathhew Seal, Saul N. Faust, Dan Owen, Ruth Ensom, Sarah M. McKay, Mariya Shaji, Rachel Schranz, Prita Rughnani, Amutha Anpananthar, Susan Liebeschuetz, Anna R. Riddell, Divya Divakaran, Louise Han, Nosheen Khalid, Ivone Lancoma Malcolm, Jessica Schofield, Teresa Simagan, Mark John Peters, Alasdair Bamford, Lauran O’Neill, Nazima Pathan, Esther Daubney, Deborah L. White, Melissa Heightman, Sarah H. Eisen, Terry Yvonne Segal, Lucy Wellings, Simon B. Drysdale, Nicole Branch, Lisa Hamzah, Heather Jarman, Lisa Capozzi, Emma Gardiner, Robert J. Moots, Magda Nasher, Anita Hanson, Michelle Linforth, Séan O’Riordan, Donna R. Ellis, Akash Deep, Iván Caro, Fiona Shackley, Arianna Bellini, Stuart Gormley, Samira Neshat, Barnaby R. Scholefield, Ceri Robbins, Helen Winmill, Stephane C. Paulus, Andrew John Pollard, Mark Anthony, Sarah Hopton, Danielle Miller, Zoë J. Oliver, Sally Beer, Bryony Ward, Shrijana Shrestha, Meeru Gurung, Puja Amatya, Bhishma Pokhrel, Sanjeev Man Bijukchhe, Madhav Chandra Gautam, Sarah Kelly, Peter John O’Reilly, Sonu Shrestha, Antonio Murillo Salas, Fernando Álvez González, Sonia Ares Gómez, Xabier Bello, Mirian Ben García, Fernando Caamaño Viña, Sandra Carnota, María José Currás-Tuala, Ana Dacosta Urbieta, Carlos Durán Suárez, Isabel Ferreirós-Vidal, Luisa García Vicente, Alberto Gómez‐Carballa, José Gómez Rial, Pilar Leboráns Iglesias, Narmeen Mallah, José María Martinón, Belén Mosquera Pérez Sánchez, Jacobo Pardo‐Seco, Sara Pischedda, Sara Rey Vázquez, Irene Rivero Calle, Carmen Rodrı́guez-Tenreiro, Lorenzo Redondo-Collazo, Sonia Serén Fernández, Marisol Vilas Iglesias, Enitan D. Carrol, Elizabeth Cocklin, Rebecca Beckley, Abbey Bracken, Ceri Evans, Aakash Khanijau, Rebecca Lenihan, Nadia Lewis-Burke, Karen Newall, Sam T. Romaine, Jennifer Whitbread, Maria N Tsolia, Irini Eleftheriou, Νikos Spyridis, Maria Tambouratzi, Despoina Maritsi, Antonios Marmarinos, Marietta Xagorari, Lourida Panagiota, Pefanis Aggelos, Akinosoglou Karolina, Gogos Charalambos, Maragos Markos, Voulgarelis Michalis, Stergiou Ioanna, Marieke Emonts, Emma Lim, John T. Isaacs, Kathryn Bell, Stephen Crulley, Daniel Fabián, Evelyn Thomson, Diane Wallia, Caroline Miller, Ashley G. Bell, Fabian Johannes Stanislaus van der Velden, Geoff Shenton, David Price, Owen Treloar, Daisy Thomas, Pablo Rojo, Cristina Epalza, Serena Villaverde, Sonia Márquez, Manuel Gijón, Fátima Machín, Laura Cabello, Irene Hernández, Lourdes Gutiérrez, Ángela Manzanares, Taco Willem Kuijpers, Martijn van de Kuip, A. Marceline van Furth, J. Merlijn van den Berg, Giske Biesbroek, Floris Verkuil, Carlijn van der Zee, Dasja Pajkrt, Michaël Boele van Hensbroek, Dieneke Schonenberg, Mariken P. Gruppen, Sietse Quirijn Nagelkerke, MachielH Jansen, Ines Goetschalckx, Lorenza Romani, Maia De Luca, Sara Chiurchiù, Costanza Tripiciano, Stefania Mercadante, Clementien L. Vermont, Henriëtte A. Moll, Dorine Borensztajn, Nienke N. Hagedoorn, Chantal D. Tan, Joany M. Zachariasse, Willem A. Dik, Ching‐Fen Shen, Dace Zavadska, Sniedze Laivacuma, Aleksandra Rudzate, Diana Stoldere, Arta Bārzdiņa, Elza Anna Bārzdiņa, Monta Madelāne, Dagne Grãvele, Dace Svilz, Romain Basmaci, Noémie Lachaume, Pauline Bories, RajaBen Tkhayat, Laura Chériaux, Juraté Davoust, Kim-Thanh Ong, Marie Cotillon, Thibault de Groc, Sébastien Lê, Nathalie Vergnault, Hélène See, Laure Cohen, Alice de Tugny, Névéna Danékova, Marine Mommert-Tripon, Karen Brengel‐Pesce, Marko Pokorn, Mojca Kolnik, Tadej Avčin, Tanja Avramoska, Natalija Bahovec, Petra Bogovič, Lidija Kitanovski, Mirijam Nahtigal, Lea Papst, Tina Plankar Srovin, Franc Strle, Katarina Vincek, Michiel van der Flier, Wim J. E. Tissing, Roelie M. Wösten‐van Asperen, Sebastiaan J. Vastert, Daniel Claes Vijlbrief, Louis Bont, Coco R. Beudeker, Philipp Agyeman, Luregn Jan Schlapbach, Christoph Aebi, Mariama Usman, Stefanie Schlüchter, Verena Wyss, Nina Schöbi, Elisa Zimmermann, Marion Meier, Kathrin Weber, Colin G. Fink, Marie A Voice, Leo A Calvo-Bado, Michael Steele, Jennifer Holden, Andrew Taylor, Ronan Calvez, Catherine S. Davies, B. J. K. Evans, Jake Stevens, Peter Matthews, Kyle Billing, Werner Zenz, Alexander Binder, Benno Kohlmaier, Daniela S. Kohlfürst, Nina A. Schweintzger, Christoph Zurl, Susanne Hösele, Manuel Leitner, Lena Pölz, Alexandra Mihaela Rusu, Glorija Rajic, Bianca Stoiser, Martina Strempfl, Manfred G. Sagmeister, Sebastian Bauchinger, Martin Benesch, Astrid Ceolotto, Ernst Eber, Siegfried Gallistl, Harald Haidl, Almuthe Christine Hauer, Christa Hude, Andreas Kapper, Markus Keldorfer, Sabine Löffler, Tobias Niedrist, Heidemarie Pilch, Andreas Pfleger, Klaus Pfurtscheller, Siegfried Rödl, Andrea Skrabl‐Baumgartner, Volker Strenger, Elmar Wallner, Maike K. Tauchert, Shunmay Yeung, Manuel Dewez, David Bath, Elizabeth J. A. Fitchett, Fiona V Cresswell, Effua Abigail Usuf, Kalifa Bojang, Anna Roca, Isatou Sarr, Momodou Saidykhan, Ebrahim Ndure, Ulrich von Both, Laura Kolberg, Patricia Schmied, Ioanna Mavridi, Irene Alba‐Alejandre, Nikolaus Alexander Haas, Esther M. Maier, Sabrina Juranek, Tobias F. Feuchtinger, Katharina Danhauser, Matthias Griese, Matthias Kappler, Eberhard Lurz, Sebastian Schroepf, Florian Hoffmann, Karl Reiter, Carola Schoen, Éric Giannoni, Martin Stocker, Klara Maria Posfay-Barbe, Ulrich Heininger, Sara Bernhard‐Stirnemann, Anita Niederer-Loher, Christian R. Kahlert, Giancarlo Natalucci, Christa Relly, Christoph T. Berger, Thomas Riedel

8Citations signalées, ce qui n’est pas une note de qualité

12Institutions déclarées

4Pays d’affiliation déclarés

Rattachement africain : gb, at, es, us. Niveau de preuve : code pays fourni par la source.

Le résumé fourni par la source

Importance: Cardiac dysfunction and myocarditis have emerged as serious complications of multisystem inflammatory syndrome in children (MIS-C) and vaccines against SARS-CoV-2. Understanding the role of autoantibodies in these conditions is essential for guiding MIS-C management and vaccination strategies in children. Objective: To investigate the presence of anticardiac autoantibodies in MIS-C or COVID-19 vaccine-induced myocarditis. Design, Setting, and Participants: This diagnostic study included children with acute MIS-C or acute vaccine myocarditis, adults with myocarditis or inflammatory cardiomyopathy, healthy children prior to the COVID-19 pandemic, and healthy COVID-19 vaccinated adults. Participants were recruited into research studies in the US, United Kingdom, and Austria starting January 2021. Immunoglobulin G (IgG), IgM, and IgA anticardiac autoantibodies were identified with immunofluorescence staining of left ventricular myocardial tissue from 2 human donors treated with sera from patients and controls. Secondary antibodies were fluorescein isothiocyanate-conjugated antihuman IgG, IgM, and IgA. Images were taken for detection of specific IgG, IgM, and IgA deposits and measurement of fluorescein isothiocyanate fluorescence intensity. Data were analyzed through March 10, 2023. Main Outcomes and Measures: IgG, IgM and IgA antibody binding to cardiac tissue. Results: By cohort, there were a total of 10 children with MIS-C (median [IQR] age, 10 [13-14] years; 6 male), 10 with vaccine myocarditis (median age, 15 [14-16] years; 10 male), 8 adults with myocarditis or inflammatory cardiomyopathy (median age, 55 [46-63] years; 6 male), 10 healthy pediatric controls (median age, 8 [13-14] years; 5 male), and 10 healthy vaccinated adults (all older than 21 years, 5 male). No antibody binding above background was observed in human cardiac tissue treated with sera from pediatric patients with MIS-C or vaccine myocarditis. One of the 8 adult patients with myocarditis or cardiomyopathy had positive IgG staining with raised fluorescence intensity (median [IQR] intensity, 11 060 [10 223-11 858] AU). There were no significant differences in median fluorescence intensity in all other patient cohorts compared with controls for IgG (MIS-C, 6033 [5834-6756] AU; vaccine myocarditis, 6392 [5710-6836] AU; adult myocarditis or inflammatory cardiomyopathy, 5688 [5277-5990] AU; healthy pediatric controls, 6235 [5924-6708] AU; healthy vaccinated adults, 7000 [6423-7739] AU), IgM (MIS-C, 3354 [3110-4043] AU; vaccine myocarditis, 3843 [3288-4748] AU; healthy pediatric controls, 3436 [3313-4237] AU; healthy vaccinated adults, 3543 [2997-4607] AU) and IgA (MIS-C, 3559 [2788-4466] AU; vaccine myocarditis, 4389 [2393-4780] AU; healthy pediatric controls, 3436 [2425-4077] AU; healthy vaccinated adults, 4561 [3164-6309] AU). Conclusions and Relevance: This etiological diagnostic study found no evidence of antibodies from MIS-C and COVID-19 vaccine myocarditis serum binding cardiac tissue, suggesting that the cardiac pathology in both conditions is unlikely to be driven by direct anticardiac antibody-mediated mechanisms.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.

Le contrôle bibliographique ouvert

DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.

Titre Crossref: Evaluation of Autoantibody Binding to Cardiac Tissue in Multisystem Inflammatory Syndrome in Children and COVID-19 Vaccination–Induced Myocarditis
Date Crossref: 18/05/2023
Éditeur: American Medical Association (AMA)
Type: journal-article

Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.

Où se fait cette recherche

Imperial College London Department of Infectious Disease pays non établi dans la notice
Université ou école supérieure
National Health Service pays non établi dans la notice
Établissement de santé
Harefield Hospital pays non établi dans la notice
Établissement de santé
Medical University of Graz Department of Cardiology pays non établi dans la notice
Université ou école supérieure
BioTechMed-Graz pays non établi dans la notice
Structure de recherche
Instituto de Salud Carlos III Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES) pays non établi dans la notice
Organisme public
Complejo Hospitalario Universitario de Santiago pays non établi dans la notice
Établissement de santé
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias pays non établi dans la notice
Structure de recherche
Instituto de Investigación Sanitaria de Santiago Vaccines and Infections Research Group (GENVIP) pays non établi dans la notice
Structure de recherche
Royal College of Paediatrics and Child Health pays non établi dans la notice
Université ou école supérieure
University of California San Diego pays non établi dans la notice
Université ou école supérieure
Rady Children's Hospital-San Diego pays non établi dans la notice
Établissement de santé

Department of Infectious Disease — Imperial College London, National Health Service et Harefield Hospital, avec 9 autres affiliations.

Une affiliation ne permet pas de déduire la nationalité d’un auteur.

Les sujets associés

Kawasaki Disease and Coronary ComplicationsSARS-CoV-2 and COVID-19 ResearchViral Infections and Immunology Research

Lire en accès ouvert Consulter la publication primaire Ouvrir le DOI