Application of a simple SNP detection method for mapping and association studies.

Résumé fourni par la source

We demonstrate the application of agarose-based TILLING (Targeting Induced Local Lesions IN Genomes) for mapping and association studies. Using the agarosebased TILLING procedure we were able to detect SNPs in pools of 8 DNA templates and expand the window for SNP detection by analyzing large amplicons (~3 kb). Using mapping populations and pedigrees in breeding programs, we evaluated SNP polymorphism of candidate genes showing differential expression patterns during hostpathogen interaction. We applied this technique for mapping oxalate oxidase, a candidate defense gene in RILs obtained from parental lines Sanhuangzhan 2 (SHZ-2) (indica) and Lijiangxin-tuan-heigu (LTH) (japonica). Results suggested that mismatch detection offers a simple way to track specific genes in breeding pedigrees and segregating populations without upfront investment in sequencing.

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