CAFs-derived rho-associated kinase1 mediated EMT to promote laryngeal squamous cell carcinoma metastasis
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Le résumé fourni par la source
BACKGROUND: Cancer-associated fibroblasts (CAFs) play an essential role in tumorigenesis and development of cancers. Nevertheless, the specific molecular mechanism of tumorigenesis and development in Laryngeal squamous cell carcinoma (LSCC) still unknown. METHODS: CAFs, CPFs and NFs were isolated and identified from laryngeal cancer, para-laryngeal cancer and normal tissues. Immunofluorescent staining, Rt-PCR and Western Blot were used to detect the expression of related proteins. Wound healing, migration, invasion and animal experiments were used to examine the ability of movement, migration, invasion and metastasis of LSCC. RESULTS: ROCK1, was highly expressed in CAFs and CAFs enhanced LSCC metastasis in vivo and vitro, and downregulation of ROCK1 in CAFs inhibited the migration and invasion of LSCC cells. While increasing ROCK1 expression in NFs promoted the migration and invasion of LSCC cells. Further studies revealed that epithelial-mesenchymal transition (EMT) and JAK2/STAT3/ERK1/2 pathway might play an essential role in promoting metastasis of LSCC. In addition, inhibition activity of ROCK1 or JAK2/STAT3/ERK1/2 signal molecules significantly reduced EMT and metastasis. CONCLUSIONS: CAFs-derived ROCK1 via JAK2/STAT3/ERK1/2 axis mediated EMT to promote LSCC metastasis and targeting ROCK1 might provide a potential treatment strategy for LSCC.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- CAFs-derived rho-associated kinase1 mediated EMT to promote laryngeal squamous cell carcinoma metastasis
- Date Crossref
- 16/04/2023
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Second Military Medical University Department of Otolaryngology pays non établi dans la noticeUniversité ou école supérieure
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Shanghai Jiao Tong University Department of Otolaryngology & Head and Neck Surgery pays non établi dans la noticeUniversité ou école supérieure
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Ruijin Hospital pays non établi dans la noticeÉtablissement de santé
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Ningxia Medical University Postgraduate Training Base at Shanghai Gongli Hospital pays non établi dans la noticeUniversité ou école supérieure
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Shanghai Pudong New Area Gongli Hospital pays non établi dans la noticeÉtablissement de santé
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Shanghai Ninth People's Hospital pays non établi dans la noticeÉtablissement de santé
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Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases pays non établi dans la noticeStructure de recherche
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Shanghai Jiaotong University School of Medicine Department of Otolaryngology-Head and Neck Surgery pays non établi dans la noticeUniversité ou école supérieure
Department of Otolaryngology — Second Military Medical University, Department of Otolaryngology & Head and Neck Surgery — Shanghai Jiao Tong University et Ruijin Hospital, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.