Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa
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Le résumé fourni par la source
BACKGROUND: Alfalfa (Medicago sativa L.) as an important legume plant can quickly produce adventitious roots (ARs) to form new plants by cutting. But the regulatory mechanism of AR formation in alfalfa remains unclear. RESULTS: To better understand the rooting process of alfalfa cuttings, plant materials from four stages, including initial separation stage (C stage), induction stage (Y stage), AR primordium formation stage (P stage) and AR maturation stage (S stage) were collected and used for RNA-Seq. Meanwhile, three candidate genes (SAUR, VAN3 and EGLC) were selected to explore their roles in AR formation. The numbers of differentially expressed genes (DEGs) of Y-vs-C (9,724) and P-vs-Y groups (6,836) were larger than that of S-vs-P group (150), indicating highly active in the early AR formation during the complicated development process. Pathways related to cell wall and sugar metabolism, root development, cell cycle, stem cell, and protease were identified, indicating that these genes were involved in AR production. A large number of hormone-related genes associated with the formation of alfalfa ARs have also been identified, in which auxin, ABA and brassinosteroids are thought to play key regulatory roles. Comparing with TF database, it was found that AP2/ERF-ERF, bHLH, WRKY, NAC, MYB, C2H2, bZIP, GRAS played a major regulatory role in the production of ARs of alfalfa. Furthermore, three identified genes showed significant promotion effect on AR formation. CONCLUSIONS: Stimulation of stem basal cells in alfalfa by cutting induced AR production through the regulation of various hormones, transcription factors and kinases. This study provides new insights of AR formation in alfalfa and enriches gene resources in crop planting and cultivation.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa
- Date Crossref
- 04/04/2023
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Beijing Forestry University pays non établi dans la noticeUniversité ou école supérieure
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University of Chinese Academy of Sciences pays non établi dans la noticeUniversité ou école supérieure
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National Institutes of Pharmaceutical Research and Development (China) pays non établi dans la noticeEntreprise
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The University of Western Australia pays non établi dans la noticeUniversité ou école supérieure
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Department of Agriculture and Food Western Australia pays non établi dans la noticeOrganisme public
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Inner Mongolia Agricultural University pays non établi dans la noticeUniversité ou école supérieure
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School of Grassland Science pays non établi dans la noticeUniversité ou école supérieure
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College of Life Sciences pays non établi dans la noticeUniversité ou école supérieure
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Ltd Beijing Tide Pharmaceutical Co. pays non établi dans la noticeEntreprise
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and UWA School of Agriculture and Environment The UWA Institute of Agriculture pays non établi dans la noticeUniversité ou école supérieure
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College of Grassland pays non établi dans la noticeUniversité ou école supérieure
Beijing Forestry University, University of Chinese Academy of Sciences et National Institutes of Pharmaceutical Research and Development (China), avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.