Supplementary Figure 4 from A Hexane Fraction of American Ginseng Suppresses Mouse Colitis and Associated Colon Cancer: Anti-inflammatory and Proapoptotic Mechanisms

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PDF file - 3.2MB, Inflammatory cells exposed to the whole AG extract and the Hexane Fraction of AG undergo apoptosis in vitro. A. TK6 cells were exposed to indicated doses of AG or the Hexane Fraction of AG dissolved in media for 24 hours. The Annexin V assay was performed to determine apoptosis post-treatment and analyzed using flow cytometry. Percentage of apoptotic cells are depicted in each histogram. A minimum of 10,000 cells were counted from three separate plates for each time and dose indicated. B. Primary CD4+/CD25- T-lymphocytes from untreated C57BL/6 mice exposed to indicated doses of AG or the Hexane Fraction of AG dissolved in media for 24 hours undergo apoptosis (assessed by Annexin V staining). CD4+/CD25- T-lymphocytes were isolated from the spleens of four different mice. Purified (>90% T-cell purity) non-activated or Con A-activated T lymphocytes (1 � 106) were exposed to indicated doses of AG or the Hexane Fraction of AG dissolved in media for 24 hours. The Annexin V assay was performed to determine apoptosis post-treatment and analyzed using flow cytometry. Percentage of apoptotic cells are depicted in each histogram. A minimum of 10,000 cells were counted from three separate plates (n = 3) for each time and dose indicated.

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