Supplementary Methods, Figures 1-9 from CCL2 Mediates Cross-talk between Cancer Cells and Stromal Fibroblasts That Regulates Breast Cancer Stem Cells
Résumé fourni par la source
PDF file - 111K, Methods: Includes Mammosphere formation assay, Cell transfection and reporter assays, RNAi studies, as well as Western blot analyses and xenograft data. Figures S1-S9 include the following: Marker expression in primary fibroblasts and BC cells (S1); Serial confocal images of representative mammospheres formed by PKH67-labeled BT474 cells in the presence or absence of CCL2 (S2); CCL2 expression in CAF3 cells upon CM treatments (S3); Sustained CCL2 induction in CAFs co-cultured with tumor cells (S4); Expression of CCR2 and CCR4 in BC cells (S5); HES1 and HEY1 reporter assays in BT474 cells (S6); In vitro secretase cleavage assay (S7); Representative IHC images of XP/CAF265922 xenograft tumors treated with PBS, IgG, or anti-CCL2 (S8); Representative IHC images of CCL2 and NOTCH1 in primary BC specimens (S9).
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Supplementary Methods, Figures 1-9 from CCL2 Mediates Cross-talk between Cancer Cells and Stromal Fibroblasts That Regulates Breast Cancer Stem Cells
- Date Crossref
- 30/03/2023
- Éditeur
- American Association for Cancer Research (AACR)
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude et ne compte pas comme une seconde source scientifique indépendante.