Response of elephant peripheral blood mononuclear cells when stimulated with elephant endotheliotropic herpesvirus glycoprotein B (EEHV-gB)
Rattachement africain : th, tw. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Elephant endotheliotropic herpesvirus-hemorrhagic disease (EEHV-HD) is the most highly fatal infectious disease among young Asian elephants. Despite the fact that antiviral therapy has been widely used, its therapeutic outcomes remain uncertain. Additionally, the virus has yet to be successfully cultivated in vitro in the process of develop viral envelope glycoproteins for vaccine design. The present study aims to investigate and evaluate EEHV1A glycoprotein B (gB) antigenic epitopes as potential candidates for further vaccine development. Epitopes of EEHV1A-gB were employed in in silico predictions and designed by using online antigenic predicting tools. Candidate genes were then constructed, transformed and expressed in the E. coli vectors prior to examine their potential for acceleration elephant immune responses in vitro. Elephant peripheral blood mononuclear cells (PBMCs) isolated from 16 healthy juvenile Asian elephants were investigated for their proliferative capability and cytokine responses after being stimulated with EEHV1A-gB epitopes. Exposure of elephant PBMCs to 20 µg/mL of gB for 72 h resulted in a significant proliferation of CD3 + cells when compared with the control group. Furthermore, proliferation of CD3 + cells was associated with a marked up-regulation of cytokine mRNA expression, involving IL-1β, IL-8, IL-12 and IFN-γ. It remains to be determined whether these candidate EEHV1A-gB epitopes could activate immune responses in animal models or elephants in vivo. Our potentially promising results demonstrate a degree of feasibility for the use of these gB epitopes in expanding EEHV vaccine development.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Response of elephant peripheral blood mononuclear cells when stimulated with elephant endotheliotropic herpesvirus glycoprotein B (EEHV-gB)
- Date Crossref
- 01/04/2023
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Chiang Mai University Center of Excellence in Elephant and Wildlife Research pays non établi dans la noticeUniversité ou école supérieure
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National Chung Hsing University pays non établi dans la noticeUniversité ou école supérieure
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Chulalongkorn University pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Veterinary Medicine Department of Veterinary Biosciences and Veterinary Public Health pays non établi dans la noticeUniversité ou école supérieure
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College of Veterinary Medicine Graduate Institute of Microbiology and Public Health pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Veterinary Sciences Department of Pathology pays non établi dans la noticeUniversité ou école supérieure
Center of Excellence in Elephant and Wildlife Research — Chiang Mai University, National Chung Hsing University et Chulalongkorn University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.