Study of nerve cell regeneration on nanofibers containing cerium oxide nanoparticles in a spinal cord injury model in rats
Rattachement africain : ir, Afrique du Sud. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Abstract Since the CNS is unable to repair itself via neuronal regeneration in adult mammals, alternative therapies need to be found. The use of cerium oxide nanoparticles to repair nerve damage could be a promising approach for spinal cord reconstruction. In this study, we constructed a scaffold containing cerium oxide nanoparticles (Scaffold-CeO2) and investigated the rate of nerve cell regeneration in a rat model of spinal cord injury. The scaffold of gelatin and polycaprolactone was synthesized, and a gelatin solution containing cerium oxide nanoparticles was attached to the scaffold. For the animal study, 40 male Wistar rats were randomly divided into 4 groups (n = 10): (a) Control; (b) Spinal cord injury (SCI); (c) Scaffold (SCI + scaffold without CeO2nanoparticles); (d) Scaffold-CeO2(SCI + scaffold containing CeO2nanoparticles). After creation of a hemisection SCI, scaffolds were placed at the site of injury in groups c and d, and after 7 weeks the rats were subjected to behavioral tests and then sacrificed for preparation of the spinal cord tissue to measure the expression of G-CSF, Tau and Mag proteins by Western blotting and Iba-1 protein by immunohistochemistry. The result of behavioral tests confirmed motor improvement and pain reduction in the Scaffold-CeO2group compared to the SCI group. Decreased expression of Iba-1 and higher expression of Tau and Mag in the Scaffold-CeO2group compared to the SCI group could be the result of nerve regeneration caused by the scaffold containing CeONPs as well as relief of pain symptoms. Graphical Abstract
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Study of nerve cell regeneration on nanofibers containing cerium oxide nanoparticles in a spinal cord injury model in rats
- Date Crossref
- 21/02/2023
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Saveh University of Medical Sciences Department of basic sciences pays non établi dans la noticeUniversité ou école supérieure
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Kerman University of Medical Sciences Physiology Research Center pays non établi dans la noticeUniversité ou école supérieure
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Iran University of Medical Sciences Radiation Biology Research Center pays non établi dans la noticeUniversité ou école supérieure
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University of Johannesburg University of Johannesburg, Afrique du Sud (code pays fourni par la source)Université ou école supérieure
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Faculty of Health Science Laser Research Centre University of Johannesburg, Afrique du Sud (pays nommé en fin d’affiliation)Université ou école supérieure
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Dr. Moshiri Veterinary Clinic pays non établi dans la noticeÉtablissement de santé
Department of basic sciences — Saveh University of Medical Sciences, Physiology Research Center — Kerman University of Medical Sciences et Radiation Biology Research Center — Iran University of Medical Sciences, avec 3 autres affiliations. Pays d’affiliation : Afrique du Sud.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.