Does the loss of diadromy imply the loss of salinity tolerance? A gene expression study with replicate nondiadromous populations of Galaxias maculatus
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Le résumé fourni par la source
The recurrent colonization of freshwater habitats and subsequent loss of diadromy is a major ecological transition that has been reported in many ancestrally diadromous fishes. Such residency is often accompanied by a loss of tolerance to seawater. The amphidromous Galaxias maculatus has repeatedly colonized freshwater streams with evidence that freshwater-resident populations exhibit stark differences in their tolerance to higher salinities. Here, we used transcriptomics to gain insight into the mechanisms contributing to reduced tolerance to higher salinities in freshwater resident populations. We conducted an acute salinity challenge (0 ppt to 23-25 ppt) and measured osmoregulatory ability (muscle water content) over 48 h in three populations: diadromous, saltwater intolerant resident (Toltén), and saltwater tolerant resident (Valdivia). RNA sequencing of the gills identified genes that were differentially expressed in association with the salinity change and associated with the loss of saltwater tolerance in the Toltén population. Key genes associated with saltwater acclimation were characterized in diadromous G. maculatus individuals, some of which were also expressed in the saltwater tolerant resident population (Valdivia). We found that some of these "saltwater acclimation" genes, including the cystic fibrosis transmembrane conductance regulator gene (CFTR), were not significantly upregulated in the saltwater intolerant resident population (Toltén), suggesting a potential mechanism for the loss of tolerance to higher salinities. As the suite of differentially expressed genes in the diadromous-resident comparison differed between freshwater populations, we hypothesize that diadromy loss results in unique evolutionary trajectories due to drift, so the loss of diadromy does not necessarily lead to a loss in upper salinity tolerance.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Does the loss of diadromy imply the loss of salinity tolerance? A gene expression study with replicate nondiadromous populations of <i>Galaxias maculatus</i>
- Date Crossref
- 06/02/2023
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Dalhousie University Department of Biology pays non établi dans la noticeUniversité ou école supérieure
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University of Concepción pays non établi dans la noticeUniversité ou école supérieure
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Centro de Ciencias Ambientales EULA-Chile de la Universidad de Concepción pays non établi dans la noticeStructure de recherche
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Millennium Institute of Oceanography pays non établi dans la noticeStructure de recherche
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Saint Mary's University Department of Biology pays non établi dans la noticeUniversité ou école supérieure
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Departamento de Sistemas Acuáticos pays non établi dans la noticeInstitution
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Departamento de Zoología pays non établi dans la noticeInstitution
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Instituto Milenio de Oceanografía (IMO) Universidad de Concepción Concepción Chile pays non établi dans la noticeStructure de recherche
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Departamento de Ingeniería Civil pays non établi dans la noticeInstitution
Department of Biology — Dalhousie University, University of Concepción et Centro de Ciencias Ambientales EULA-Chile de la Universidad de Concepción, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.