Neuronal and astrocytic tetraploidy is increased in drug‐resistant epilepsy
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Le résumé fourni par la source
AIMS: Epilepsy is one of the most prevalent neurological diseases. A third of patients with epilepsy remain drug-resistant. The exact aetiology of drug-resistant epilepsy (DRE) is still unknown. Neuronal tetraploidy has been associated with neuropathology. The aim of this study was to assess the presence of tetraploid neurons and astrocytes in DRE. METHODS: For that purpose, cortex, hippocampus and amygdala samples were obtained from patients subjected to surgical resection of the epileptogenic zone. Post-mortem brain tissue of subjects without previous records of neurological, neurodegenerative or psychiatric diseases was used as control. RESULTS: The percentage of tetraploid cells was measured by immunostaining of neurons (NeuN) or astrocytes (S100β) followed by flow cytometry analysis. The results were confirmed by image cytometry (ImageStream X Amnis System Cytometer) and with an alternative astrocyte biomarker (NDRG2). Statistical comparison was performed using univariate tests. A total of 22 patients and 10 controls were included. Tetraploid neurons and astrocytes were found both in healthy individuals and DRE patients in the three brain areas analysed: cortex, hippocampus and amygdala. DRE patients presented a higher number of tetraploid neurons (p = 0.020) and astrocytes (p = 0.002) in the hippocampus than controls. These results were validated by image cytometry. CONCLUSIONS: We demonstrated the presence of both tetraploid neurons and astrocytes in healthy subjects as well as increased levels of both cell populations in DRE patients. Herein, we describe for the first time the presence of tetraploid astrocytes in healthy subjects. Furthermore, these results provide new insights into epilepsy, opening new avenues for future treatment.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Neuronal and astrocytic tetraploidy is increased in drug‐resistant epilepsy
- Date Crossref
- 10/01/2023
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Hospital Universitario de La Princesa Department of Neurology pays non établi dans la noticeÉtablissement de santé
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Instituto de Medicina Genómica pays non établi dans la noticeStructure de recherche
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Instituto Cajal Department of Molecular pays non établi dans la noticeStructure de recherche
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Universidad de Zaragoza pays non établi dans la noticeUniversité ou école supérieure
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Universidad San Pablo CEU pays non établi dans la noticeUniversité ou école supérieure
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NIMGenetics Genómica y Medicina S.L. Madrid Spain pays non établi dans la noticeInstitution
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Department of Clinical Pharmacology pays non établi dans la noticeÉtablissement de santé
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Department of Clinical Analysis pays non établi dans la noticeÉtablissement de santé
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Fisiología y Medicina Legal y Forense University of Zaragoza Zaragoza Spain Departamento de Farmacología pays non établi dans la noticeUniversité ou école supérieure
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Departamento de Ciencias Farmacéuticas y de la Salud pays non établi dans la noticeInstitution
Department of Neurology — Hospital Universitario de La Princesa, Instituto de Medicina Genómica et Department of Molecular — Instituto Cajal, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.