Prevalence and Genetic Analysis of Resistance Mechanisms of Linezolid-Nonsusceptible Enterococci in a Tertiary Care Hospital Examined via Whole-Genome Sequencing
Rattachement africain : kr, us, Ghana. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
(1) Background: Linezolid plays an important role in the treatment of invasive infections caused by vancomycin-resistant enterococci after its introduction to clinical practice. However, a detailed examination of linezolid-nonsusceptible enterococci (LNSE) is required. In this study, we attempted to analyze the mechanisms of LNSE strains isolated from a tertiary care hospital. (2) Methods: From 2019 to 2020, 18 Enterococcus faecalis, 14 E. faecium, and 2 E. gallinarum clinical isolates were collected at Severance Hospital. Agar dilution was performed to evaluate precise linezolid minimum inhibitory concentrations (MICs). Short-read whole-genome sequencing (WGS) was used to analyze resistance determinants. (3) Results: The presence of the optrA gene was likely the primary resistance mechanism in these three species, typically demonstrating a MIC value of 8 μg/mL. The co-existence of the cfr(D) and poxtA2 gene was the second major mechanism, primarily predicting a phenotype showing intermediate susceptibility to linezolid. G2576U mutation on 23S rRNA was only found in E. faecium; it mediated the most significant increase in linezolid MIC. (4) Conclusion: This is the first report examining poxtA2–cfr(D) co-harboring clinical enterococcal isolates in Korea and demonstrating the poxtA EF9F6-harboring clinical E. gallinarum strain worldwide. The comparison with resistance-gene-containing fragments in the isolates obtained from different countries and different sources demonstrated the spread of linezolid-resistance genes and suggested the possibility of foodborne transmission.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Prevalence and Genetic Analysis of Resistance Mechanisms of Linezolid-Nonsusceptible Enterococci in a Tertiary Care Hospital Examined via Whole-Genome Sequencing
- Date Crossref
- 14/11/2022
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Yonsei University Department of Laboratory Medicine and Research Institute of Bacterial Resistance pays non établi dans la noticeUniversité ou école supérieure
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Cedars-Sinai Medical Center pays non établi dans la noticeÉtablissement de santé
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Tamale Teaching Hospital Tamale Teaching Hospital, Ghana (code pays fourni par la source)Établissement de santé
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Department of Laboratory Services Tamale Teaching Hospital, Ghana (pays nommé en fin d’affiliation)Structure de recherche
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Seoul Clinical Laboratories Academy pays non établi dans la noticeÉtablissement de santé
Department of Laboratory Medicine and Research Institute of Bacterial Resistance — Yonsei University, Cedars-Sinai Medical Center et Tamale Teaching Hospital (Tamale Teaching Hospital, Ghana), avec 2 autres affiliations. Pays d’affiliation : Ghana.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.