A fijiviral nonstructural protein triggers cell death in plant and bacterial cells via its transmembrane domain
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Le résumé fourni par la source
Southern rice black-streaked dwarf virus (SRBSDV; Fijivirus, Reoviridae) has become a threat to cereal production in East Asia in recent years. Our previous cytopathologic studies have suggested that SRBSDV induces a process resembling programmed cell death in infected tissues that results in distinctive growth abnormalities. The viral product responsible for the cell death, however, remains unknown. Here P9-2 protein, but not its RNA, was shown to induce cell death in Escherichia coli and plant cells when expressed either locally with a transient expression vector or systemically using a heterologous virus. Both computer prediction and fluorescent assays indicated that the viral nonstructural protein was targeted to the plasma membrane (PM) and further modification of its subcellular localization abolished its ability to induce cell death, indicating that its PM localization was required for the cell death induction. P9-2 was predicted to harbour two transmembrane helices within its central hydrophobic domain. A series of mutation assays further showed that its central transmembrane hydrophobic domain was crucial for cell death induction and that its conserved F90, Y101, and L103 amino acid residues could play synergistic roles in maintaining its ability to induce cell death. Its homologues in other fijiviruses also induced cell death in plant and bacterial cells, implying that the fijiviral nonstructural protein may trigger cell death by targeting conserved cellular factors or via a highly conserved mechanism.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A fijiviral nonstructural protein triggers cell death in plant and bacterial cells via its transmembrane domain
- Date Crossref
- 28/10/2022
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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ZheJiang Academy of Agricultural Sciences pays non établi dans la noticeOrganisation à but non lucratif
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Zhejiang Normal University pays non établi dans la noticeUniversité ou école supérieure
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Zhejiang Sci-Tech University pays non établi dans la noticeUniversité ou école supérieure
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Zhejiang University Analysis Center of Agrobiology and Environmental Sciences pays non établi dans la noticeUniversité ou école supérieure
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Laboratory of Virology pays non établi dans la noticeStructure de recherche
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College of Chemistry and Life Science pays non établi dans la noticeUniversité ou école supérieure
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College of Life Science Zhejiang Sci‐Tech University Hangzhou China pays non établi dans la noticeUniversité ou école supérieure
ZheJiang Academy of Agricultural Sciences, Zhejiang Normal University et Zhejiang Sci-Tech University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.