A Deletion Encompassing the Furin Cleavage Site in the Spike Encoding Gene Does Not Alter SARS-CoV-2 Replication in Lung Tissues of Mink and Neutralization by Convalescent Human Serum Samples
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Le résumé fourni par la source
SARS-CoV-2 has been shown to lose the furin polybasic cleavage site (FCS) following adaptation on cell culture. Deletion occurring in this region, which may include also the FCS flanking regions, seem not to affect virus replication in vitro; however, a chimeric SARS-CoV-2 virus without the sole FCS motif has been associated with lower virulence in mice and lower neutralization values. Moreover, SARS-CoV-2 virus lacking the FCS was shed to lower titers from experimentally infected ferrets and was not transmitted to cohoused sentinel animals, unlike wild-type virus. In this study, we investigated the replication kinetics and cellular tropism of a SARS-CoV-2 isolate carrying a 10-amino acid deletion in the spike protein spanning the FCS in lung ex vivo organ cultures of mink. Furthermore, we tested the neutralization capabilities of human convalescent SARS-CoV-2 positive serum samples against this virus. We showed that this deletion did not significantly hamper neither ex vivo replication nor neutralization activity by convalescent serum samples. This study highlights the importance of the preliminary phenotypic characterization of emerging viruses in ex vivo models and demonstrates that mink lung tissues are permissive to the replication of a mutant form of SARS-CoV-2 showing a deletion spanning the FCS. Notably, we also highlight the need for sequencing viral stocks before any infection study as large deletions may occur leading to the misinterpretation of results.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A Deletion Encompassing the Furin Cleavage Site in the Spike Encoding Gene Does Not Alter SARS-CoV-2 Replication in Lung Tissues of Mink and Neutralization by Convalescent Human Serum Samples
- Date Crossref
- 06/10/2022
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale pays non établi dans la noticeStructure de recherche
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University of Teramo pays non établi dans la noticeUniversité ou école supérieure
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Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata pays non établi dans la noticeStructure de recherche
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University of Bari Aldo Moro Department of Veterinary Medicine pays non établi dans la noticeUniversité ou école supérieure
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Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise pays non établi dans la noticeInstitution
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Faculty of Veterinary Medicine pays non établi dans la noticeUniversité ou école supérieure
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, University of Teramo et Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.