Differential Sequencing by Mass Spectrometry

Le résumé fourni par la source

The publication of the human genome sequence in 2001 and the completion of hundreds of thousands of virus, bacterial, archaeal, and eukaryotic genomes by 2020 affirm the success of worldwide genome sequencing projects. The availability of new reference sequence information of various organisms is continuously growing. This chapter describes the use of mass spectrometry (MS) to rapidly identify and localize variable genomic regions based on reference sequences, thus facilitating high-throughput differential sequencing with a gamut of applications. Analysis of nucleic acids by MS has mainly been accomplished by two common soft ionization techniques—electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) MS. The use of MALDI coupled with time-of-flight MS (MALDI-TOF MS) has become one of the leading technologies for high-throughput analysis and high-fidelity measurement of nucleic acid sequence variations. MALDI-TOF MS of nucleic acids after base-specific cleavage improves the odds of differential sequence analysis and sets a significant milestone in the field of comparative genomics and genetics.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.